BMRB Entry 19043

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PDB ID: 2m5a
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19043
MolProbity Validation Chart

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Title:
Protein A binding by an engineered Affibody molecule
Deposition date:
2013-02-19
Original release date:
2013-08-14
Authors:
Hard, Torleif
Citation:

Citation: Lindborg, Malin; Dubnovitsky, Anatoly; Olesen, Kenneth; Bjorkman, Tomas; Abrahmsen, Lars; Feldwisch, Joachim; Hard, Torleif. "High-affinity binding to staphylococcal protein A by an engineered dimeric Affibody molecule."  Protein Eng. Des. Sel. 26, 635-644 (2013).
PubMed: 23924760

Assembly members:

Assembly members:
Z_domain, polymer, 58 residues, 6648.390 Da.
ZpA963, polymer, 58 residues, 6394.143 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Z_domain: VDNKFNKEQQNAFYEILHLP NLNEEQRNAFIQSLKDDPSQ SANLLAEAKKLNDAQAPK
ZpA963: VDNKFNKETQEASWEIFTLP NLNGRQVAAFISSLLDDPSQ SANLLAEAKKLNDAQAPK

Data sets:
Data typeCount
13C chemical shifts507
15N chemical shifts135
1H chemical shifts835

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Z_domain1
2ZpA9632

Entities:

Entity 1, Z_domain 58 residues - 6648.390 Da.

1   VALASPASNLYSPHEASNLYSGLUGLNGLN
2   ASNALAPHETYRGLUILELEUHISLEUPRO
3   ASNLEUASNGLUGLUGLNARGASNALAPHE
4   ILEGLNSERLEULYSASPASPPROSERGLN
5   SERALAASNLEULEUALAGLUALALYSLYS
6   LEUASNASPALAGLNALAPROLYS

Entity 2, ZpA963 58 residues - 6394.143 Da.

1   VALASPASNLYSPHEASNLYSGLUTHRGLN
2   GLUALASERTRPGLUILEPHETHRLEUPRO
3   ASNLEUASNGLYARGGLNVALALAALAPHE
4   ILESERSERLEULEUASPASPPROSERGLN
5   SERALAASNLEULEUALAGLUALALYSLYS
6   LEUASNASPALAGLNALAPROLYS

Related Database Links:

BMRB 4023
PDB 1H0T 1LP1 1Q2N 2M5A 2SPZ 4GWP 4GWQ
DBJ BAC76617 BAC76619 BAL27690 BAL27694 BAL27697
EMBL CAA49867 CAA65431
GB AAA72944 AAA73085 AAB00807 AAR10383 AAR10384
REF WP_052996913

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