BMRB Entry 19034

Name: NMR Structure of the third RNA Recognition Motif (RRM) of U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2
Published: 2013-03-21

Click here to enlarge.

PDB ID: 2m52
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19034
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR Structure of the third RNA Recognition Motif (RRM) of U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2

Deposition date:

2013-02-12

Original release date:

2013-03-21

Authors:

Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt

Citation:

Citation: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt. "NMR Structure of the third RNA Recognition Motif (RRM) of U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2" .

Assembly members:

Assembly members:
U2AF, polymer, 105 residues, 12033.749 Da.

Natural source:

Natural source: Common Name: House mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PspeedET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
U2AF: GGHPTEVLCLMNMVLPEELL DDEEYEEIVEDVRDECSKYG LVKSIEIPRPVDGVEVPGCG KIFVEFTSVFDCQKAMQGLT GRKFANRVVVTKYCDPDSYH RRDFW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	367
15N chemical shifts	102
1H chemical shifts	721

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	U2AF	1

Entities:

Entity 1, U2AF 105 residues - 12033.749 Da.

1

GLY

HIS

PRO

THR

GLU

VAL

LEU

CYS

LEU

2

MET

ASN

MET

VAL

LEU

PRO

GLU

LEU

3

ASP

GLU

TYR

GLU

ILE

VAL

GLU

4

ASP

VAL

ARG

ASP

GLU

CYS

SER

LYS

TYR

GLY

5

LEU

VAL

LYS

SER

ILE

GLU

ILE

PRO

ARG

PRO

6

VAL

ASP

GLY

VAL

GLU

VAL

PRO

GLY

CYS

GLY

7

LYS

ILE

PHE

VAL

GLU

PHE

THR

SER

VAL

PHE

8

ASP

CYS

GLN

LYS

ALA

MET

GLN

GLY

LEU

THR

9

GLY

ARG

LYS

PHE

ALA

ASN

ARG

VAL

10

THR

LYS

TYR

CYS

ASP

PRO

ASP

SER

TYR

HIS

11

ARG

ASP

PHE

TRP

Samples:

sample_1: U2AF, [U-98% 13C; U-98% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 5 mM; DTT 8 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1298 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
4D-HACANH-APSY	sample_1	isotropic	sample_conditions_1
5D-CBCACONH-APSY	sample_1	isotropic	sample_conditions_1
5D-HACACONH-APSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

CYANA, G ntert P. - structure solution

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

TOPSPIN v3.1, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

j-UNIO, Herrmann, Guntert and Wuthrich - chemical shift assignment, peak picking, structure solution

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

PDB	1O0P 1OPI 2M0G 2M52 3V4M 4FXW 4Z2X
DBJ	BAC37309 BAG70075 BAG70201 BAI45669
EMBL	CAA45409 CAA45874 CAA45875
GB	AAH07487 AAH08740 AAH30574 AAH43071 AAH89996
PRF	1805352A
REF	NP_001012496 NP_001068804 NP_001192160 NP_001302733 NP_009210
SP	P26368 P26369
TPG	DAA19368
AlphaFold	P26368 P26369