BMRB Entry 19030

Name: Analysis of the structural and molecular basis of voltage-sensitive sodium channel inhibition by the spider toxin, Huwentoxin-IV ( -TRTX-Hh2a).
Published: 2013-06-17

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PDB ID: 2m4z
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19030
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Analysis of the structural and molecular basis of voltage-sensitive sodium channel inhibition by the spider toxin, Huwentoxin-IV ( -TRTX-Hh2a).

Deposition date:

2013-02-12

Original release date:

2013-06-17

Authors:

Gibbs, Al

Citation:

Citation: Minassian, Natali; Gibbs, Alan; Shih, Amy; Liu, Yi; Neff, Robert; Sutton, Steven; Mirzadegan, Tara; Connor, Judith; Fellows, Ross; Husovsky, Matthew; Nelson, Serena; Hunter, Michael; Flinspach, Mack; Wickenden, Alan. "Analysis of the Structural and Molecular Basis of Voltage-sensitive Sodium Channel Inhibition by the Spider Toxin Huwentoxin-IV (-TRTX-Hh2a)." J. Biol. Chem. 288, 22707-22720 (2013).
PubMed: 23760503

Assembly members:

Assembly members:
Huwentoxin-IV, polymer, 35 residues, 4005.742 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Homo Sapien Vector: pCDNA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Huwentoxin-IV: ECLEIFKACNPSNDQCCKSS KLVCSRKTRACKYQI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	191

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Huwentoxin-IV	1

Entities:

Entity 1, Huwentoxin-IV 35 residues - 4005.742 Da.

1

GLU

CYS

LEU

GLU

ILE

PHE

LYS

ALA

CYS

ASN

2

PRO

SER

ASN

ASP

GLN

CYS

LYS

SER

3

LYS

LEU

VAL

CYS

SER

ARG

LYS

THR

ARG

ALA

4

CYS

LYS

TYR

GLN

ILE

Samples:

sample_1: Huwentoxin-IV 2 mM; sodium phosphate 20 mM; sodium azide 150 uM; EDTA, [U-2H], 100 uM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Bruker Avance 950 MHz

BMRB	19026
PDB	1MB6 2M4X 2M4Z
GB	AAP33074 ABY77744 ABY77746
SP	P83303
AlphaFold	P83303