BMRB Entry 19000

Name: Solution NMR structure of VV2_0175 from Vibrio vulnificus, NESG target VnR1 and CSGID target IDP91333
Published: 2013-02-20

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PDB ID: 2m4e
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19000
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of VV2_0175 from Vibrio vulnificus, NESG target VnR1 and CSGID target IDP91333

Deposition date:

2013-02-04

Original release date:

2013-02-20

Authors:

Wu, Bin; Yee, Adelinda; Houliston, Scott; Lemak, Alexander; Garcia, Maite; Savchenko, Alexei; Arrowsmith, Cheryl

Citation:

Citation: Wu, Bin; Yee, Adelinda; Houliston, Scott; Lemak, Alexander; Garcia, Maite; Savchenko, Alexei; Arrowsmith, Cheryl. "Solution NMR structure of VV2_0175 from Vibrio vulnificus, NESG target VnR1 and CSGID target IDP91333" .

Assembly members:

Assembly members:
VV2_0175, polymer, 110 residues, 9530.360 Da.

Natural source:

Natural source: Common Name: g-proteobacteria Taxonomy ID: 672 Superkingdom: Bacteria Kingdom: not available Genus/species: Vibrio vulnificus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMCSG7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VV2_0175: MHHHHHHSSGVDLGTENLYF QSNAMSKTAKLNNEEKLVKK ALEIGGKMAKMQGFDLPQSP QPVRVKAVYLFLVDAKQIAP LPDSKLDGANIKHRLALWIH AALPDNDPLK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	356
15N chemical shifts	71
1H chemical shifts	609

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	VV2_0175	1

Entities:

Entity 1, VV2_0175 110 residues - 9530.360 Da.

residues M-23 - A0 represents histag

1	MET	HIS	HIS	HIS	HIS	HIS	HIS	SER	SER	GLY
2	VAL	ASP	LEU	GLY	THR	GLU	ASN	LEU	TYR	PHE
3	GLN	SER	ASN	ALA	MET	SER	LYS	THR	ALA	LYS
4	LEU	ASN	ASN	GLU	GLU	LYS	LEU	VAL	LYS	LYS
5	ALA	LEU	GLU	ILE	GLY	GLY	LYS	MET	ALA	LYS
6	MET	GLN	GLY	PHE	ASP	LEU	PRO	GLN	SER	PRO
7	GLN	PRO	VAL	ARG	VAL	LYS	ALA	VAL	TYR	LEU
8	PHE	LEU	VAL	ASP	ALA	LYS	GLN	ILE	ALA	PRO
9	LEU	PRO	ASP	SER	LYS	LEU	ASP	GLY	ALA	ASN
10	ILE	LYS	HIS	ARG	LEU	ALA	LEU	TRP	ILE	HIS
11	ALA	ALA	LEU	PRO	ASP	ASN	ASP	PRO	LEU	LYS

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D TOCSY-HSQC	sample_1	isotropic	sample_conditions_1

PDB	2M4E
DBJ	BAC96709
GB	AAO07148 ADV88695 AIL72739 ALM73137 EWS70635
REF	WP_011081156 WP_046030100 WP_047110511

BMRB Entry 19000

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	HIS	HIS	HIS	HIS	HIS	HIS	SER	SER	GLY
2	VAL	ASP	LEU	GLY	THR	GLU	ASN	LEU	TYR	PHE
3	GLN	SER	ASN	ALA	MET	SER	LYS	THR	ALA	LYS
4	LEU	ASN	ASN	GLU	GLU	LYS	LEU	VAL	LYS	LYS
5	ALA	LEU	GLU	ILE	GLY	GLY	LYS	MET	ALA	LYS
6	MET	GLN	GLY	PHE	ASP	LEU	PRO	GLN	SER	PRO
7	GLN	PRO	VAL	ARG	VAL	LYS	ALA	VAL	TYR	LEU
8	PHE	LEU	VAL	ASP	ALA	LYS	GLN	ILE	ALA	PRO
9	LEU	PRO	ASP	SER	LYS	LEU	ASP	GLY	ALA	ASN
10	ILE	LYS	HIS	ARG	LEU	ALA	LEU	TRP	ILE	HIS
11	ALA	ALA	LEU	PRO	ASP	ASN	ASP	PRO	LEU	LYS

1	MET	HIS	HIS	HIS	HIS	HIS	HIS	SER	SER	GLY
2	VAL	ASP	LEU	GLY	THR	GLU	ASN	LEU	TYR	PHE
3	GLN	SER	ASN	ALA	MET	SER	LYS	THR	ALA	LYS
4	LEU	ASN	ASN	GLU	GLU	LYS	LEU	VAL	LYS	LYS
5	ALA	LEU	GLU	ILE	GLY	GLY	LYS	MET	ALA	LYS
6	MET	GLN	GLY	PHE	ASP	LEU	PRO	GLN	SER	PRO
7	GLN	PRO	VAL	ARG	VAL	LYS	ALA	VAL	TYR	LEU
8	PHE	LEU	VAL	ASP	ALA	LYS	GLN	ILE	ALA	PRO
9	LEU	PRO	ASP	SER	LYS	LEU	ASP	GLY	ALA	ASN
10	ILE	LYS	HIS	ARG	LEU	ALA	LEU	TRP	ILE	HIS
11	ALA	ALA	LEU	PRO	ASP	ASN	ASP	PRO	LEU	LYS

1	MET	HIS	HIS	HIS	HIS	HIS	HIS	SER	SER	GLY
2	VAL	ASP	LEU	GLY	THR	GLU	ASN	LEU	TYR	PHE
3	GLN	SER	ASN	ALA	MET	SER	LYS	THR	ALA	LYS
4	LEU	ASN	ASN	GLU	GLU	LYS	LEU	VAL	LYS	LYS
5	ALA	LEU	GLU	ILE	GLY	GLY	LYS	MET	ALA	LYS
6	MET	GLN	GLY	PHE	ASP	LEU	PRO	GLN	SER	PRO
7	GLN	PRO	VAL	ARG	VAL	LYS	ALA	VAL	TYR	LEU
8	PHE	LEU	VAL	ASP	ALA	LYS	GLN	ILE	ALA	PRO
9	LEU	PRO	ASP	SER	LYS	LEU	ASP	GLY	ALA	ASN
10	ILE	LYS	HIS	ARG	LEU	ALA	LEU	TRP	ILE	HIS
11	ALA	ALA	LEU	PRO	ASP	ASN	ASP	PRO	LEU	LYS