BMRB Entry 18947
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18947
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Citation: Zheng, Jianting; Fage, Christopher; Demeler, Borries; Hoffman, David; Keatinge-Clay, Adrian. "The missing linker: a dimerization motif located within polyketide synthase modules." ACS Chem. Biol. 8, 1263-1270 (2013).
PubMed: 23489133
Assembly members:
SpnDE1, polymer, 72 residues, 7800 Da.
Natural source: Common Name: High GC Gram+ Taxonomy ID: 60894 Superkingdom: Bacteria Kingdom: not available Genus/species: Saccharopolyspora spinosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b
Entity Sequences (FASTA):
SpnDE1: GSHMALRESSAGDMGRRVEA
KFWGAVEHEDVESLARVLGI
VDDGAAVDSLRSALPVLAGW
QRTRTTESIMDQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 238 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 406 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SpnDE1 | 1 |
Entities:
Entity 1, SpnDE1 72 residues - 7800 Da.
Residues 1-3 are remnants of a thrombin-cleaved hexahistidine tag
| 1 | GLY | SER | HIS | MET | ALA | LEU | ARG | GLU | SER | SER | ||||
| 2 | ALA | GLY | ASP | MET | GLY | ARG | ARG | VAL | GLU | ALA | ||||
| 3 | LYS | PHE | TRP | GLY | ALA | VAL | GLU | HIS | GLU | ASP | ||||
| 4 | VAL | GLU | SER | LEU | ALA | ARG | VAL | LEU | GLY | ILE | ||||
| 5 | VAL | ASP | ASP | GLY | ALA | ALA | VAL | ASP | SER | LEU | ||||
| 6 | ARG | SER | ALA | LEU | PRO | VAL | LEU | ALA | GLY | TRP | ||||
| 7 | GLN | ARG | THR | ARG | THR | THR | GLU | SER | ILE | MET | ||||
| 8 | ASP | GLN |
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