BMRB Entry 18939

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18939
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution Structure of C-terminal AbrB
Deposition date:
2013-01-04
Original release date:
2013-01-24
Authors:
Olson, Andrew; Tucker, Ashley; Cavanagh, John
Citation:

Citation: Olson, Andrew. "Structure of Full Length AbrB from Bacillus Subtilis"  Structure ., .-..

Assembly members:

Assembly members:
C-terminal AbrB, chain 1, polymer, 46 residues, 4996.743 Da.

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal AbrB, chain 1: MNCMTCQVTGEVSDDNLKLA GGKLVLSKEGAEQIISEIQN QLQNLK

Data sets:
Data typeCount
13C chemical shifts168
15N chemical shifts43
1H chemical shifts277

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal AbrB, chain 11
2C-terminal AbrB, chain 21

Entities:

Entity 1, C-terminal AbrB, chain 1 46 residues - 4996.743 Da.

1   METASNCYSMETTHRCYSGLNVALTHRGLY
2   GLUVALSERASPASPASNLEULYSLEUALA
3   GLYGLYLYSLEUVALLEUSERLYSGLUGLY
4   ALAGLUGLNILEILESERGLUILEGLNASN
5   GLNLEUGLNASNLEULYS

Samples:

water: potassium phosphate 10 mM; DTT 1 mM; potassium chloride 15 mM; EDTA 1 mM; D2O 10%; H2O 90%; C-term_AbrB 1 mM

d20: DTT 1 mM; potassium phosphate 10 mM; potassium chloride 15 mM; EDTA 1 mM; D2O 100%; C-term_AbrB 1 mM

sample_conditions_1: ionic strength: 0.015 M; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCwaterisotropicsample_conditions_1
2D 1H-13C HSQCwaterisotropicsample_conditions_1
3D CBCA(CO)NHwaterisotropicsample_conditions_1
3D C(CO)NHwaterisotropicsample_conditions_1
3D HNCOwaterisotropicsample_conditions_1
3D HNCACBwaterisotropicsample_conditions_1
3D H(CCO)NHwaterisotropicsample_conditions_1
3D HCCH-TOCSYd20isotropicsample_conditions_1
3D HNHAwaterisotropicsample_conditions_1
3D 1H-15N NOESYwaterisotropicsample_conditions_1
3D 1H-13C NOESYd20isotropicsample_conditions_1
3D 1H-13C NOESY aromaticd20isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, data analysis

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA05272 BAI83483 BAM48968 BAM56238 GAK78652
EMBL CAA31307 CAA43955 CAB11813 CBI41163 CCF03572
GB AAA22195 ABS72486 ACB37468 ADM36096 ADV94967
PRF 1715209A
REF NP_387918 WP_003218316 WP_003226760 WP_004264711 WP_014469596
SP P08874
AlphaFold P08874

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks