BMRB Entry 18874

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PDB ID: 2m1r
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18874
MolProbity Validation Chart

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Title:
PHD domain of ING4 N214D mutant
Deposition date:
2012-12-04
Original release date:
2012-12-13
Authors:
Blanco, Francisco
Citation:

Citation: Blanco, Francisco; Palmero, Ignacio. "Functional impact of cancer-associated mutations in the tumor suppressor protein ING4"  Carinogenesis 31, 1932-1938 (2010).
PubMed: 20705953

Assembly members:

Assembly members:
ING4_PHD_mutant_N214D, polymer, 63 residues, 7316.4 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ING4_PHD_mutant_N214D: MDMPVDPNEPTYCLCHQVSY GEMIGCDDPDCSIEWFHFAC VGLTTKPRGKWFCPRCSQER KKK

Data sets:
Data typeCount
15N chemical shifts61
1H chemical shifts368

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ING4 PHD mutant N214D1
2Zn ion, 12
3Zn ion, 22

Entities:

Entity 1, ING4 PHD mutant N214D 63 residues - 7316.4 Da.

Residue Met187 is not native. It comes from the cloning of the fragment and expression in E coli

1   METASPMETPROVALASPPROASNGLUPRO
2   THRTYRCYSLEUCYSHISGLNVALSERTYR
3   GLYGLUMETILEGLYCYSASPASPPROASP
4   CYSSERILEGLUTRPPHEHISPHEALACYS
5   VALGLYLEUTHRTHRLYSPROARGGLYLYS
6   TRPPHECYSPROARGCYSSERGLNGLUARG
7   LYSLYSLYS

Entity 2, Zn ion, 1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

UNP Q9UNL4
PDB 2K1J 2M1R 2PNX 2VNF
AlphaFold Q9H3J0

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