BMRB Entry 18845
Click here to enlarge.
PDB ID: 4ch0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18845
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Amrane, Samir; Mackereth, Cameron. "Protein chemical shift assignments of the unbound and RNA-bound forms of the alternative splicing factor SUP-12 from C. elegans." Biomol. NMR Assignments ., .-. (2013).
PubMed: 23334698
Assembly members:
SUP-12, polymer, 97 residues, 10815.1 Da.
Natural source: Common Name: Nematode Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-His1a
Entity Sequences (FASTA):
SUP-12: GAMGSRDTMFTKIFVGGLPY
HTSDKTLHEYFEQFGDIEEA
VVITDRNTQKSRGYGFVTMK
DRASAERACKDPNPIIDGRK
ANVNLAYLGAKPRTNVQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 431 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 668 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SUP-12 | 1 |
Entities:
Entity 1, SUP-12 97 residues - 10815.1 Da.
The N-terminal Gly-Ala-Met remains following removal of the His6-tag by TEV potease
| 1 | GLY | ALA | MET | GLY | SER | ARG | ASP | THR | MET | PHE | ||||
| 2 | THR | LYS | ILE | PHE | VAL | GLY | GLY | LEU | PRO | TYR | ||||
| 3 | HIS | THR | SER | ASP | LYS | THR | LEU | HIS | GLU | TYR | ||||
| 4 | PHE | GLU | GLN | PHE | GLY | ASP | ILE | GLU | GLU | ALA | ||||
| 5 | VAL | VAL | ILE | THR | ASP | ARG | ASN | THR | GLN | LYS | ||||
| 6 | SER | ARG | GLY | TYR | GLY | PHE | VAL | THR | MET | LYS | ||||
| 7 | ASP | ARG | ALA | SER | ALA | GLU | ARG | ALA | CYS | LYS | ||||
| 8 | ASP | PRO | ASN | PRO | ILE | ILE | ASP | GLY | ARG | LYS | ||||
| 9 | ALA | ASN | VAL | ASN | LEU | ALA | TYR | LEU | GLY | ALA | ||||
| 10 | LYS | PRO | ARG | THR | ASN | VAL | GLN |
Related Database Links:
| UNP | O45189 H2L051 |
| BMRB | 11541 18846 19653 |
| PDB | 2MGZ 2RU3 4CH0 4CH1 4CIO |
| EMBL | CCD71425 CCD71429 |
| GB | KIH66793 |
| REF | NP_001129938 NP_508674 |
| AlphaFold | H2L051 O45189 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks