BMRB Entry 18825

Title:
Complex structure of C-terminal CFTR peptide and extended PDZ1 domain from NHERF1.
Deposition date:
2012-11-06
Original release date:
2013-04-22
Authors:
Bhattacharya, Shibani; Ju, Jeong; Cowburn, David; Bu, Zimei
Citation:

Citation: Bhattacharya, Shibani; Ju, Jeong Ho; Orlova, Natalia; Khajeh, Jahan Ali; Cowburn, David; Bu, Zimei. "Ligand-Induced Dynamic Changes in Extended PDZ Domains from NHERF1."  J. Mol. Biol. 425, 2509-2528 (2013).
PubMed: 23583913

Assembly members:

Assembly members:
PDZ1, polymer, 117 residues, 12822.731 Da.
CFTR, polymer, 5 residues, 632.693 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET151/D-TOPO

Data sets:
Data typeCount
13C chemical shifts481
15N chemical shifts116
1H chemical shifts832

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1extended PDZ1 domain from NHERF11
2C-terminal CFTR peptide2

Entities:

Entity 1, extended PDZ1 domain from NHERF1 117 residues - 12822.731 Da.

1   GLYILEASPPROPHETHRMETLEUPROARG
2   LEUCYSCYSLEUGLULYSGLYPROASNGLY
3   TYRGLYPHEHISLEUHISGLYGLULYSGLY
4   LYSLEUGLYGLNTYRILEARGLEUVALGLU
5   PROGLYSERPROALAGLULYSALAGLYLEU
6   LEUALAGLYASPARGLEUVALGLUVALASN
7   GLYGLUASNVALGLULYSGLUTHRHISGLN
8   GLNVALVALSERARGILEARGALAALALEU
9   ASNALAVALARGLEULEUVALVALASPPRO
10   GLUTHRASPGLUGLNLEUGLNLYSLEUGLY
11   VALGLNVALARGGLUGLULEULEUARGALA
12   GLNGLUALAPROGLYGLNALA

Entity 2, C-terminal CFTR peptide 5 residues - 632.693 Da.

1   GLNASPTHRARGLEU

Related Database Links:

BMRB 18824
PDB 1G9O 2M0T 2M0U

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks