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PDB ID: 2m0u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18825
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Bhattacharya, Shibani; Ju, Jeong Ho; Orlova, Natalia; Khajeh, Jahan Ali; Cowburn, David; Bu, Zimei. "Ligand-Induced Dynamic Changes in Extended PDZ Domains from NHERF1." J. Mol. Biol. 425, 2509-2528 (2013).
PubMed: 23583913
Assembly members:
PDZ1, polymer, 117 residues, 12822.731 Da.
CFTR, polymer, 5 residues, 632.693 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET151/D-TOPO
Entity Sequences (FASTA):
PDZ1: GIDPFTMLPRLCCLEKGPNG
YGFHLHGEKGKLGQYIRLVE
PGSPAEKAGLLAGDRLVEVN
GENVEKETHQQVVSRIRAAL
NAVRLLVVDPETDEQLQKLG
VQVREELLRAQEAPGQA
CFTR: QDTRL
Data type | Count |
13C chemical shifts | 481 |
15N chemical shifts | 116 |
1H chemical shifts | 832 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | extended PDZ1 domain from NHERF1 | 1 |
2 | C-terminal CFTR peptide | 2 |
Entity 1, extended PDZ1 domain from NHERF1 117 residues - 12822.731 Da.
1 | GLY | ILE | ASP | PRO | PHE | THR | MET | LEU | PRO | ARG | ||||
2 | LEU | CYS | CYS | LEU | GLU | LYS | GLY | PRO | ASN | GLY | ||||
3 | TYR | GLY | PHE | HIS | LEU | HIS | GLY | GLU | LYS | GLY | ||||
4 | LYS | LEU | GLY | GLN | TYR | ILE | ARG | LEU | VAL | GLU | ||||
5 | PRO | GLY | SER | PRO | ALA | GLU | LYS | ALA | GLY | LEU | ||||
6 | LEU | ALA | GLY | ASP | ARG | LEU | VAL | GLU | VAL | ASN | ||||
7 | GLY | GLU | ASN | VAL | GLU | LYS | GLU | THR | HIS | GLN | ||||
8 | GLN | VAL | VAL | SER | ARG | ILE | ARG | ALA | ALA | LEU | ||||
9 | ASN | ALA | VAL | ARG | LEU | LEU | VAL | VAL | ASP | PRO | ||||
10 | GLU | THR | ASP | GLU | GLN | LEU | GLN | LYS | LEU | GLY | ||||
11 | VAL | GLN | VAL | ARG | GLU | GLU | LEU | LEU | ARG | ALA | ||||
12 | GLN | GLU | ALA | PRO | GLY | GLN | ALA |
Entity 2, C-terminal CFTR peptide 5 residues - 632.693 Da.
1 | GLN | ASP | THR | ARG | LEU |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks