BMRB Entry 18811
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PDB ID: 2m0m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18811
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Gao, Zhenwei; Lin, Zhi; Huang, Weidong; Lai, Chong Cheong; Fan, Jing-Song; Yang, Daiwen. "Structural characterization of minor ampullate spidroin domains and their distinct roles in fibroin solubility and fiber formation." PLoS ONE 8, e56142-e56142 (2013).
PubMed: 23418525
Assembly members:
Spidroin, polymer, 107 residues, 10505.787 Da.
Natural source: Common Name: Spiders Taxonomy ID: 171624 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Nephila antipodiana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-32a derived
Entity Sequences (FASTA):
Spidroin: VGTTVASTTSRLSTAEASSR
ISTAASTLVSGGYLNTAALP
SVIADLFAQVGASSPGVSDS
EVLIQVLLEIVSSLIHILSS
SSVGQVDFSSVGSSAAAVGQ
SMQVVMG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 313 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 678 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Spidroin_1 | 1 |
| 2 | Spidroin_2 | 1 |
Entities:
Entity 1, Spidroin_1 107 residues - 10505.787 Da.
| 1 | VAL | GLY | THR | THR | VAL | ALA | SER | THR | THR | SER | ||||
| 2 | ARG | LEU | SER | THR | ALA | GLU | ALA | SER | SER | ARG | ||||
| 3 | ILE | SER | THR | ALA | ALA | SER | THR | LEU | VAL | SER | ||||
| 4 | GLY | GLY | TYR | LEU | ASN | THR | ALA | ALA | LEU | PRO | ||||
| 5 | SER | VAL | ILE | ALA | ASP | LEU | PHE | ALA | GLN | VAL | ||||
| 6 | GLY | ALA | SER | SER | PRO | GLY | VAL | SER | ASP | SER | ||||
| 7 | GLU | VAL | LEU | ILE | GLN | VAL | LEU | LEU | GLU | ILE | ||||
| 8 | VAL | SER | SER | LEU | ILE | HIS | ILE | LEU | SER | SER | ||||
| 9 | SER | SER | VAL | GLY | GLN | VAL | ASP | PHE | SER | SER | ||||
| 10 | VAL | GLY | SER | SER | ALA | ALA | ALA | VAL | GLY | GLN | ||||
| 11 | SER | MET | GLN | VAL | VAL | MET | GLY |
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