BMRB Entry 18800

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18800

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Title:
Backbone assignments of the apo and Zn(II) protoporphyrin IX-bound states of the soluble form of rat heme oxygenase-1
Deposition date:
2012-10-22
Original release date:
2015-05-07
Authors:
Harada, Erisa; Sugishima, Masakazu; Harada, Jiro; Noguchi, Masato; Fukuyama, Keiichi; Sugase, Kenji
Citation:

Citation: Harada, Erisa; Sugishima, Masakazu; Harada, Jiro; Noguchi, Masato; Fukuyama, Keiichi; Sugase, Kenji. "Backbone assignments of the apo and Zn(II) protoporphyrin IX-bound states of the soluble form of rat heme oxygenase-1"  Biomol. NMR Assign. 9, 197-200 (2015).
PubMed: 25209143

Assembly members:

Assembly members:
HO-1_complex, polymer, 232 residues, 26800 Da.
PROTOPORPHYRIN IX CONTAINING ZN, non-polymer, 626.051 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HO-1_complex: MERPQLDSMSQDLSEALKEA TKEVHIRAENSEFMRNFQKG QVSREGFKLVMASLYHIYTA LEEEIERNKQNPVYAPLYFP EELHRRAALEQDMAFWYGPH WQEAIPYTPATQHYVKRLHE VGGTHPELLVAHAYTRYLGD LSGGQVLKKIAQKAMALPSS GEGLAFFTFPSIDNPTKFKQ LYRARMNTLEMTPEVKHRVT EEAKTAFLLNIELFEELQAL LTEEHKDQSPSQ

Data sets:
Data typeCount
13C chemical shifts666
15N chemical shifts214
1H chemical shifts214

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HO-1_complex1
2zinc_protoporphyrin_IX2

Entities:

Entity 1, HO-1_complex 232 residues - 26800 Da.

1   METGLUARGPROGLNLEUASPSERMETSER
2   GLNASPLEUSERGLUALALEULYSGLUALA
3   THRLYSGLUVALHISILEARGALAGLUASN
4   SERGLUPHEMETARGASNPHEGLNLYSGLY
5   GLNVALSERARGGLUGLYPHELYSLEUVAL
6   METALASERLEUTYRHISILETYRTHRALA
7   LEUGLUGLUGLUILEGLUARGASNLYSGLN
8   ASNPROVALTYRALAPROLEUTYRPHEPRO
9   GLUGLULEUHISARGARGALAALALEUGLU
10   GLNASPMETALAPHETRPTYRGLYPROHIS
11   TRPGLNGLUALAILEPROTYRTHRPROALA
12   THRGLNHISTYRVALLYSARGLEUHISGLU
13   VALGLYGLYTHRHISPROGLULEULEUVAL
14   ALAHISALATYRTHRARGTYRLEUGLYASP
15   LEUSERGLYGLYGLNVALLEULYSLYSILE
16   ALAGLNLYSALAMETALALEUPROSERSER
17   GLYGLUGLYLEUALAPHEPHETHRPHEPRO
18   SERILEASPASNPROTHRLYSPHELYSGLN
19   LEUTYRARGALAARGMETASNTHRLEUGLU
20   METTHRPROGLUVALLYSHISARGVALTHR
21   GLUGLUALALYSTHRALAPHELEULEUASN
22   ILEGLULEUPHEGLUGLULEUGLNALALEU
23   LEUTHRGLUGLUHISLYSASPGLNSERPRO
24   SERGLN

Entity 2, zinc_protoporphyrin_IX - C34 H32 N4 O4 Zn - 626.051 Da.

1   ZNH

Related Database Links:

PDB 3VYZ 1DVE 1IRM 1IVJ 1IX3 1IX4 1J02 1J2C 1UBB 1ULX 1VGI 2DY5 2E7E 2ZVU 3I9T 3I9U 3WKT 4G7L 4G7P 4G7T 4G7U 4G8P 4G8U 4G8W 4G98 4G99 4MEC
BMRB 18798 18799
GB AAA41346 AAH91164 ADD84876 EDL87376
REF NP_036712
SP P06762
AlphaFold P06762

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks