BMRB Entry 18792

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PDB ID: 2m03
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18792
MolProbity Validation Chart

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Title:
Solution structure of BCL-xL determined with selective isotope labelling of I,L,V sidechains
Deposition date:
2012-10-19
Original release date:
2013-01-22
Authors:
Viacava Follis, Ariele; Royappa, Grace; Kriwacki, Richard
Citation:

Citation: Follis, Ariele Viacava; Chipuk, Jerry; Fisher, John; Yun, Mi-Kyung; Grace, Christy; Nourse, Amanda; Baran, Katherine; Ou, Li; Min, Lie; White, Stephen; Green, Douglas; Kriwacki, Richard. "PUMA binding induces partial unfolding within BCL-xL to disrupt p53 binding and promote apoptosis."  Nat. Chem. Biol. 9, 163-168 (2013).
PubMed: 23340338

Assembly members:

Assembly members:
BCL-xL_apo, polymer, 181 residues, 20252.496 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BCL-xL_apo: MSMAMSQSNRELVVDFLSYK LSQKGYSWSQFSDVEENRTE APEGTESEAVKQALREAGDE FELRYRRAFSDLTSQLHITP GTAYQSFEQVVNELFRDGVN WGRIVAFFSFGGALCVESVD KEMQVLVSRIAAWMATYLND HLEPWIQENGGWDTFVELYG NNAAAESRKGQERLEHHHHH H

Data sets:
Data typeCount
13C chemical shifts536
15N chemical shifts171
1H chemical shifts343

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1BCL-xL_apo1

Entities:

Entity 1, BCL-xL_apo 181 residues - 20252.496 Da.

Wild-type Human protein with truncation of 22 C terminal residues and disordered loop between alpha helices 1 and 2

1   METSERMETALAMETSERGLNSERASNARG
2   GLULEUVALVALASPPHELEUSERTYRLYS
3   LEUSERGLNLYSGLYTYRSERTRPSERGLN
4   PHESERASPVALGLUGLUASNARGTHRGLU
5   ALAPROGLUGLYTHRGLUSERGLUALAVAL
6   LYSGLNALALEUARGGLUALAGLYASPGLU
7   PHEGLULEUARGTYRARGARGALAPHESER
8   ASPLEUTHRSERGLNLEUHISILETHRPRO
9   GLYTHRALATYRGLNSERPHEGLUGLNVAL
10   VALASNGLULEUPHEARGASPGLYVALASN
11   TRPGLYARGILEVALALAPHEPHESERPHE
12   GLYGLYALALEUCYSVALGLUSERVALASP
13   LYSGLUMETGLNVALLEUVALSERARGILE
14   ALAALATRPMETALATHRTYRLEUASNASP
15   HISLEUGLUPROTRPILEGLNGLUASNGLY
16   GLYTRPASPTHRPHEVALGLULEUTYRGLY
17   ASNASNALAALAALAGLUSERARGLYSGLY
18   GLNGLUARGLEUGLUHISHISHISHISHIS
19   HIS

Related Database Links:

GB CAA80661

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