BMRB Entry 18704

Title:
human Siglec5 Carbohydrate Recognition Domain Chemical Shifts
Deposition date:
2012-09-06
Original release date:
2012-09-07
Authors:
Barb, Adam; Prestegard, James
Citation:

Citation: Barb, Adam; Wang, Xu; Prestegard, James. "NMR chemical shift mapping of refolded human sialic-acid-binding immunoglobulin-like lectin 5 binding to complex carbohydrates"  .

Assembly members:

Assembly members:
Siglec5, polymer, 143 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28b

Data sets:
Data typeCount
13C chemical shifts330
15N chemical shifts110
1H chemical shifts110

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Siglec51

Entities:

Entity 1, Siglec5 143 residues - Formula weight is not available

This construct encodes residues P19 to A141 of human siglec5; from Uniprot DB: O15389

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   PROVALTYRGLULEUGLNVALGLNLYSSER
4   VALTHRVALGLNGLUGLYLEUALAVALLEU
5   VALPROCYSSERPHESERTYRPROTRPARG
6   SERTRPTYRSERSERPROPROLEUTYRVAL
7   TYRTRPPHEARGASPGLYGLUILEPROTYR
8   TYRALAGLUVALVALALATHRASNASNPRO
9   ASPARGARGVALLYSPROGLUTHRGLNGLY
10   ARGPHEARGLEULEUGLYASPVALGLNLYS
11   LYSASNCYSSERLEUSERILEGLYASPALA
12   ARGMETGLUASPTHRGLYSERTYRPHEPHE
13   ARGVALGLUARGGLYARGASPVALLYSTYR
14   SERTYRGLNGLNASNLYSLEUASNLEUGLU
15   VALTHRALA

Related Database Links:

PDB 2ZG1 2ZG2 2ZG3
DBJ BAG60544
GB AAB70703 AAD50978 AAF87846 AAH29896 AAK77223
REF NP_001092082 NP_003821 XP_006723021 XP_011524598 XP_011524599
SP O15389 Q08ET2
AlphaFold O15389 Q08ET2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks