BMRB Entry 18696

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18696

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Title:
1H, 13C and 15N resonance assignment of the N-terminal domain of human lysyl aminoacyl tRNA synthetase
Deposition date:
2012-08-31
Original release date:
2012-10-18
Authors:
Liu, Sheng; Tsang, Pearl
Citation:

Citation: Liu, Sheng; Decker, Aaron; Howell, Mike; Caperelli, Carol; Tsang, Pearl. "1H, 13C and 15N resonance assignment of the N-terminal domain of human lysyl aminoacyl tRNA synthetase."  Biomol. NMR Assignments 7, 289-292 (2013).
PubMed: 23065336

Assembly members:

Assembly members:
rmodN, polymer, 76 residues, 8311 Da.
rmodN_isoD62, polymer, 76 residues, 8312 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rmodN: MAAVQAAEVKVDGSEPKLSK NELKRRLKAEKKVAEKEAKQ KELSEKQLSQATAAATNHTT DNGVLPETGGHHHHHH
rmodN_isoD62: MAAVQAAEVKVDGSEPKLSK NELKRRLKAEKKVAEKEAKQ KELSEKQLSQATAAATNHTT DDGVLPETGGHHHHHH

Data sets:
Data typeCount
13C chemical shifts574
15N chemical shifts143
1H chemical shifts660

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1rmodN1
2rmodN_isoD622

Entities:

Entity 1, rmodN 76 residues - 8311 Da.

1   METALAALAVALGLNALAALAGLUVALLYS
2   VALASPGLYSERGLUPROLYSLEUSERLYS
3   ASNGLULEULYSARGARGLEULYSALAGLU
4   LYSLYSVALALAGLULYSGLUALALYSGLN
5   LYSGLULEUSERGLULYSGLNLEUSERGLN
6   ALATHRALAALAALATHRASNHISTHRTHR
7   ASPASNGLYVALLEUPROGLUTHRGLYGLY
8   HISHISHISHISHISHIS

Entity 2, rmodN_isoD62 76 residues - 8312 Da.

N62 deamidate into isoD62

1   METALAALAVALGLNALAALAGLUVALLYS
2   VALASPGLYSERGLUPROLYSLEUSERLYS
3   ASNGLULEULYSARGARGLEULYSALAGLU
4   LYSLYSVALALAGLULYSGLUALALYSGLN
5   LYSGLULEUSERGLULYSGLNLEUSERGLN
6   ALATHRALAALAALATHRASNHISTHRTHR
7   ASPASPGLYVALLEUPROGLUTHRGLYGLY
8   HISHISHISHISHISHIS

Related Database Links:

BMRB 18697 18697
DBJ BAA06688 BAA22084 BAC86604 BAG09591 BAA06688 BAA22084 BAC86604 BAG09591
GB AAH04132 ABM83227 ABM86426 AIC54646 EAW95620 AAH04132 ABM83227 ABM86426 AIC54646 EAW95620
REF NP_005539 XP_003260017 XP_003829594 XP_004058057 XP_009249230 NP_005539 XP_003260017 XP_003829594 XP_009249230 XP_511115
SP Q15046 Q15046
AlphaFold Q15046 Q15046

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CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks