BMRB Entry 18688

Title:
Backbone amide chemical shifts of gp78 RING bound to Ube2g2:G2BR
Deposition date:
2012-08-30
Original release date:
2013-08-26
Authors:
Das, Ranabir; Linag, Yuhe; Mariano, Jennifer; Li, Jess; Huang, Tao; King, Aaren; Weissman, Allan; Ji, Xinhua; Byrd, R. Andrew
Citation:

Citation: Das, Ranabir; Liang, Yu-He; Mariano, Jennifer; Li, Jess; Huang, Tao; King, Aaren; Tarasov, Sergey; Weissman, Allan; Ji, Xinhua; Byrd, R. Andrew. "Allosteric regulation of E2:E3 interactions promote a processive ubiquitination machine."  EMBO J. 32, 2504-2516 (2013).
PubMed: 23942235

Assembly members:

Assembly members:
entity_1, polymer, 156 residues, 17556.293 Da.
entity_2, polymer, 27 residues, 3365.939 Da.
entity_3, polymer, 58 residues, 6473.385 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Data sets:
Data typeCount
15N chemical shifts55
1H chemical shifts55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22
3entity_33
4ZINC ION_14
5ZINC ION_24

Entities:

Entity 1, entity_1 156 residues - 17556.293 Da.

1   ALAGLYTHRALALEULYSARGLEUMETALA
2   GLUTYRLYSGLNLEUTHRLEUASNPROPRO
3   GLUGLYILEVALALAGLYPROMETASNGLU
4   GLUASNPHEPHEGLUTRPGLUALALEUILE
5   METGLYPROGLUASPTHRCYSPHEGLUPHE
6   GLYVALPHEPROALAILELEUSERPHEPRO
7   LEUASPTYRPROLEUSERPROPROLYSMET
8   ARGPHETHRCYSGLUMETPHEHISPROASN
9   ILETYRPROASPGLYARGVALCYSILESER
10   ILELEUHISALAPROGLYSERALAGLUARG
11   TRPSERPROVALGLNSERVALGLULYSILE
12   LEULEUSERVALVALSERMETLEUALAGLU
13   PROASNASPGLUSERGLYALAASNVALASP
14   ALASERLYSMETTRPARGASPASPARGGLU
15   GLNPHETYRLYSILEALALYSGLNILEVAL
16   GLNLYSSERLEUGLYLEU

Entity 2, entity_2 27 residues - 3365.939 Da.

1   SERALAASPGLUARGGLNARGMETLEUVAL
2   GLNARGLYSASPGLULEULEUGLNGLNALA
3   ARGLYSARGPHELEUASNLYS

Entity 3, entity_3 58 residues - 6473.385 Da.

1   ALAVALALATHRPROGLUGLULEUALAVAL
2   ASNASNASPASPCYSALAILECYSTRPASP
3   SERMETGLNALAALAARGLYSLEUPROCYS
4   GLYHISLEUPHEHISASNSERCYSLEUARG
5   SERTRPLEUGLUGLNASPTHRSERCYSPRO
6   THRCYSARGMETSERLEUASNILE

Entity 4, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: gp78RING, [U-100% 13C; U-100% 15N], 0.7 – 1 mM; TRIS 50 mM; TCEP 2 mM; sodium azide 0.2 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

SPARKY, Goddard - chemical shift assignment, data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CNS, Alexandre Bonvin - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

REF XP_007486190 NP_001135 NP_001267243 XP_001091030 XP_002761010 XP_003263117 NP_001039439 NP_001135 NP_001267243 NP_001271666 NP_035917
PDB
DBJ BAE01277 BAK63135 BAE01277 BAE34049 BAE41974 BAE87377 BAK63135
GB AAA36671 AAA79362 AAD56722 AAH17043 AAH56869 AAD56721 AAD56722 AAH17043 AAH34538 AAH40338
SP Q9UKV5 Q9R049 Q9UKV5
BMRB 18677
TPG DAA20037
AlphaFold Q9UKV5 Q9R049 Q9UKV5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks