BMRB Entry 18673

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PDB ID: 2lxf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18673
MolProbity Validation Chart

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Title:
Solution NMR structure of a potential acylphosphatase from Giardia lamblia, Seattle Structural Genomics Center for Infectious Disease target GilaA.01396.a
Deposition date:
2012-08-20
Original release date:
2012-09-25
Authors:
Buchko, Garry
Citation:

Citation: Buchko, Garry; Hewitt, Stephan; Napuli, Alberto; Van Voorhis, Wesley; Myler, Peter. "Solution structure of an acylphosphatase from Giardia lamblia, the etiological agent responsible for giardiasis"  .

Assembly members:

Assembly members:
entity, polymer, 121 residues, 13221.998 Da.

Natural source:

Natural source:   Common Name: Giardia intestinalis ATCC 50803   Taxonomy ID: 184922   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Giardia lamblia

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: AVA0421

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MAHHHHHHMGTLEAQTQGPG SMQGSMPSSSEDVTTLCYRV TGKVQGVFFRKYTKKEADAL SLVGYVTNNEDGSVSGVVQG PKEQVDAFVKYLHKGSPKSV VKKVSIHASSRVDADGFEIR R

Data sets:
Data typeCount
13C chemical shifts410
15N chemical shifts99
1H chemical shifts598

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1potential acylphosphatase from Giardia lamblia1

Entities:

Entity 1, potential acylphosphatase from Giardia lamblia 121 residues - 13221.998 Da.

Residues 1-21 are non-native residues added to the protein to facilitate purification.

1   METALAHISHISHISHISHISHISMETGLY
2   THRLEUGLUALAGLNTHRGLNGLYPROGLY
3   SERMETGLNGLYSERMETPROSERSERSER
4   GLUASPVALTHRTHRLEUCYSTYRARGVAL
5   THRGLYLYSVALGLNGLYVALPHEPHEARG
6   LYSTYRTHRLYSLYSGLUALAASPALALEU
7   SERLEUVALGLYTYRVALTHRASNASNGLU
8   ASPGLYSERVALSERGLYVALVALGLNGLY
9   PROLYSGLUGLNVALASPALAPHEVALLYS
10   TYRLEUHISLYSGLYSERPROLYSSERVAL
11   VALLYSLYSVALSERILEHISALASERSER
12   ARGVALASPALAASPGLYPHEGLUILEARG
13   ARG

Related Database Links:

PDB 2LXF
GB EDO78469 ESU35281
REF XP_001706143

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