BMRB Entry 18651

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PDB ID: 2mme
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18651
MolProbity Validation Chart

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Title:
13C and 15N chemical shifts for Shigella Flexneri MxiH Type three secretion system needle subunit
Deposition date:
2012-08-08
Original release date:
2013-02-14
Authors:
Demers, Jean-Philippe; Sgourakis, Nikolaos; Gupta, Rashmi; Loquet, Antoine; Giller, Karin; Riedel, Dietmar; Laube, Britta; Kolbe, Michael; Baker, David; Becker, Stefan; Lange, Adam
Citation:

Citation: Demers, Jean-Philippe; Sgourakis, Nikolaos; Gupta, Rashmi; Loquet, Antoine; Giller, Karin; Riedel, Dietmar; Laube, Britta; Kolbe, Michael; Baker, David; Becker, Stefan; Lange, Adam. "The Common Structural Architecture of Shigella flexneri and Salmonella typhimurium Type Three Secretion Needles"  PLoS Pathog. 9, e1003245-e1003245 (2013).
PubMed: 23555258

Assembly members:

Assembly members:
MxiH, polymer, 85 residues, 9389.3 Da.

Natural source:

Natural source:   Common Name: Shigella flexneri   Taxonomy ID: 424719   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Shigella flexneri

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MxiH: GHMSVTVPDKDWTLSSLSET FDDGTQTLQGQLTSALNALA ENPSNPQLLAEYQSKLSEYT LYRNAQSNTVKVIKDVDAAI IQNFR

Data sets:
Data typeCount
13C chemical shifts387
15N chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MxiH needle1

Entities:

Entity 1, MxiH needle 85 residues - 9389.3 Da.

The sequence employed in this study is found in S. flexneri serotype 6. After protease cleavage of the N-terminal His tag, the released MxiH protein contains the non-native N-terminal residues glycine and histidine.

1   GLYHISMETSERVALTHRVALPROASPLYS
2   ASPTRPTHRLEUSERSERLEUSERGLUTHR
3   PHEASPASPGLYTHRGLNTHRLEUGLNGLY
4   GLNLEUTHRSERALALEUASNALALEUALA
5   GLUASNPROSERASNPROGLNLEULEUALA
6   GLUTYRGLNSERLYSLEUSERGLUTYRTHR
7   LEUTYRARGASNALAGLNSERASNTHRVAL
8   LYSVALILELYSASPVALASPALAALAILE
9   ILEGLNASNPHEARG

Related Database Links:

UNP Q6XVY0_SHIFL
PDB 2MME
GB AAP79000 ACD06130 KHQ70608 KIZ60406
REF WP_012421721 WP_032324888 WP_039064401 WP_046891853 WP_047204460
AlphaFold Q6XVY0