BMRB Entry 18648

Name: APPTM V44M
Published: 2012-09-07

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PDB ID: 2lz4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18648
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

APPTM V44M

Deposition date:

2012-08-07

Original release date:

2012-09-07

Authors:

Chen, Wen; Wang, Chunyu

Citation:

Citation: Chen, Wen; Gamache, Eric; Rosenman, David; Xie, Jian; Lopez, Maria; Li, Yue-Ming; Wang, Chunyu. "Familial Alzheimer's mutations within APPTM increase A42 production by enhancing accessibility of -cleavage site." Nat. Commun. 5, 3037-3037 (2014).
PubMed: 24390130

Assembly members:

Assembly members:
V44M monomer, polymer, 32 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PetM41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
V44M monomer: GAMAKGAIIGLMVGGVVIAT MIVITLVMLKKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	144
15N chemical shifts	30
1H chemical shifts	252

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	V44M chain, 1	1
2	V44M chain, 2	1

Entities:

Entity 1, V44M chain, 1 32 residues - Formula weight is not available

1

GLY

ALA

MET

ALA

LYS

GLY

ALA

ILE

GLY

2

LEU

MET

VAL

GLY

VAL

ILE

ALA

THR

3

MET

ILE

VAL

ILE

THR

LEU

VAL

MET

LEU

LYS

4

LYS

Samples:

15N_V44M: APPTM V44M dimer, [U-100% 13C; U-100% 15N], mM; potassium chloride 0.5 mM; sodium chloride 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 7.2; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N_V44M	isotropic	sample_conditions_1
2D 1H-13C HSQC	15N_V44M	isotropic	sample_conditions_1
3D C(CO)NH	15N_V44M	isotropic	sample_conditions_1
3D HNCO	15N_V44M	isotropic	sample_conditions_1
3D HNCACB	15N_V44M	isotropic	sample_conditions_1
3D HBHA(CO)NH	15N_V44M	isotropic	sample_conditions_1
3D H(CCO)NH	15N_V44M	isotropic	sample_conditions_1
3D HCCH-TOCSY	15N_V44M	isotropic	sample_conditions_1
3D 1H-15N TOCSY	15N_V44M	isotropic	sample_conditions_1

Software:

SPARKY, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz