BMRB Entry 18632

Title:
Resonance assignment of As-p18, a structurally unusual fatty acid binding protein secreted by developing larvae of the parasitic nematode Ascaris suum
Deposition date:
2012-08-01
Original release date:
2013-02-12
Authors:
Ibanez Shimabukuro, Marina; Smith, Brian; Rey Burusco, M Florencia; Corsico, Betina; Kennedy, Malcolm; Cooper, Alan
Citation:

Citation: Ibanez-Shimabukuro, Marina; Rey-Burusco, M. Florencia; Cooper, Alan; Kennedy, Malcolm; Corsico, Betina; Smith, Brian. "Resonance assignment of As-p18, a fatty acid binding protein secreted by developing larvae of the parasitic nematode Ascaris suum."  Biomol. NMR Assignments 8, 33-36 (2014).
PubMed: 23225165

Assembly members:

Assembly members:
rAs-p18, polymer, 155 residues, 18320.5283 Da.
entity_OLA, non-polymer, 282.461 Da.

Natural source:

Natural source:   Common Name: Ascaris suum   Taxonomy ID: 6253   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ascaris suum

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pQE-30

Data typeCount
13C chemical shifts1190
15N chemical shifts300
1H chemical shifts2060

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rAs-p181
2Oleic Acid2

Entities:

Entity 1, rAs-p18 155 residues - 18320.5283 Da.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERLYSTHRLEUPROASPLYSPHELEU
3   GLYTHRPHELYSLEUGLUARGASPGLUASN
4   PHEASPGLUTYRLEULYSALAARGGLYTYR
5   GLYTRPILEMETARGGLNVALILELYSLEU
6   ALAGLYVALTHRLYSLYSPHEARGASNALA
7   ALASERGLYLYSPROASPARGTYRASPMET
8   GLUASNLEUTHRTHRLYSLYSASPTHRHIS
9   HISLYSASPTRPALALEUGLYGLUGLUPHE
10   GLNASPGLUALALEUASPSERTHRGLNHIS
11   LYSILETHRPHEASPLEULYSASPPROASN
12   THRLEUTHRGLUTHRHISILELYSVALASP
13   ASPPROTHRASPVALGLUTHRTYRGLUTYR
14   ARGARGASPGLYASPTYRLEUVALMETLYS
15   METSERTRPLYSGLYVALSERTHRSERARG
16   TYRTYRLYSLYSGLN

Entity 2, Oleic Acid - C18 H34 O2 - 282.461 Da.

1   OLA

Samples:

NC005Asp18: rAs-p18, [U-99% 13C; U-99% 15N], 400.0 mM; Sodium phosphate buffer 20.0 mM

Asp18: Oleic Acid 2000.0 mM; rAs-p18, [U-99% 13C; U-99% 15N], 500.0 mM; Sodium phosphate buffer 20.0 mM

NC_noHPLC_Asp18: rAs-p18, [U-99% 13C; U-99% 15N], 500.0 mM; Sodium phosphate buffer 60.0 mM

Asp18_noHPLC: ionic strength: 60.000 mM; pH: 7.400; pressure: 1.000 atm; temperature: 297.700 K

Asp18NC005: ionic strength: 0.020 M; pH: 7.200; pressure: 1.000 atm; temperature: 298.000 K

Asp18NC007: ionic strength: 0.020 M; pH: 7.400; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
3D HNCONC_noHPLC_Asp18isotropicAsp18_noHPLC
CBCACONH ({CA|Cca}coNH)NC_noHPLC_Asp18isotropicAsp18_noHPLC
3D HNCACBNC_noHPLC_Asp18isotropicAsp18_noHPLC
HNCACO (H[N[ca[CO]]])NC_noHPLC_Asp18isotropicAsp18_noHPLC
3D HBHA(CO)NHNC_noHPLC_Asp18isotropicAsp18_noHPLC
2D 1H-15N HSQC/HMQCNC005Asp18isotropicAsp18NC005
3D HCCH-TOCSYNC_noHPLC_Asp18isotropicAsp18_noHPLC
3D 1H-13C NOESYNC005Asp18isotropicAsp18NC005
2D 1H-13C HSQC/HMQCNC005Asp18isotropicAsp18NC005
2D 1H-15N HSQC/HMQCAsp18isotropicAsp18NC007
Arom32HD (hbCBcgcdHD)Asp18isotropicAsp18NC007
Arom33HE (hbCBcgcdceHE)Asp18isotropicAsp18NC007
3D 1H-15N NOESYAsp18isotropicAsp18NC007
mxen HCCH (hC_CH.relayed)NC005Asp18isotropicAsp18NC005
2D 1H-13C HSQC/HMQCAsp18isotropicAsp18NC007

Software:

ANALYSIS v2.0, CCPN - data analysis

CcpNmr_Entry_Completion_Interface v2.1, PDBe & CCPN - data deposition

DANGLE v1.1, Cheung & Broadhurst - dihedral angle prediction

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

GenBank AAA98565.1
GB AAA98565
SP P55776
AlphaFold P55776

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks