BMRB Entry 18595

Click here to enlarge.

PDB ID: 5uk6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR18595
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solid-state NMR 13C and 15N resonance assignments of a seven-transmembrane helical protein Anabaena Sensory Rhodopsin
Deposition date:
2012-07-16
Original release date:
2012-08-29
Authors:
Wang, Shenlin; Shi, Lichi; Brown, Leonid; Ladizhansky, Vladimir
Citation:

Citation: Wang, Shenlin; Shi, Lichi; Okitsu, Takashi; Wada, Akimori; Brown, Leonid; Ladizhansky, Vladimir. "Solid-state NMR 13C and 15N resonance assignments of a seven-transmembrane helical protein Anabaena Sensory Rhodopsin"  Biomol. NMR Assign. 7, 253-256 (2013).
PubMed: 22983928

Assembly members:

Assembly members:
ASR, polymer, 235 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Cyanobacteria   Taxonomy ID: 1172   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Anabaena variabilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMS107

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ASR: MNLESLLHWIYVAGMTIGAL HFWSLSRNPRGVPQYEYLVA MFIPIWSGLAYMAMAIDQGK VEAAGQIAHYARYIDWMVTT PLLLLSLSWTAMQFIKKDWT LIGFLMSTQIVVITSGLIAD LSERDWVRYLWYICGVCAFL IILWGIWNPLRAKTRTQSSE LANLYDKLVTYFTVLWIGYP IVWIIGPSGFGWINQTIDTF LFCLLPFFSKVGFSFLDLHG LRNLNDSRQHHHHHH

Data sets:
Data typeCount
13C chemical shifts963
15N chemical shifts223

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Anabeana sensory rhodopsin1

Entities:

Entity 1, Anabeana sensory rhodopsin 235 residues - Formula weight is not available

6X his-tagged C-terminal truncated at position 229 Anabeana sensory rhodospin

1   METASNLEUGLUSERLEULEUHISTRPILE
2   TYRVALALAGLYMETTHRILEGLYALALEU
3   HISPHETRPSERLEUSERARGASNPROARG
4   GLYVALPROGLNTYRGLUTYRLEUVALALA
5   METPHEILEPROILETRPSERGLYLEUALA
6   TYRMETALAMETALAILEASPGLNGLYLYS
7   VALGLUALAALAGLYGLNILEALAHISTYR
8   ALAARGTYRILEASPTRPMETVALTHRTHR
9   PROLEULEULEULEUSERLEUSERTRPTHR
10   ALAMETGLNPHEILELYSLYSASPTRPTHR
11   LEUILEGLYPHELEUMETSERTHRGLNILE
12   VALVALILETHRSERGLYLEUILEALAASP
13   LEUSERGLUARGASPTRPVALARGTYRLEU
14   TRPTYRILECYSGLYVALCYSALAPHELEU
15   ILEILELEUTRPGLYILETRPASNPROLEU
16   ARGALALYSTHRARGTHRGLNSERSERGLU
17   LEUALAASNLEUTYRASPLYSLEUVALTHR
18   TYRPHETHRVALLEUTRPILEGLYTYRPRO
19   ILEVALTRPILEILEGLYPROSERGLYPHE
20   GLYTRPILEASNGLNTHRILEASPTHRPHE
21   LEUPHECYSLEULEUPROPHEPHESERLYS
22   VALGLYPHESERPHELEUASPLEUHISGLY
23   LEUARGASNLEUASNASPSERARGGLNHIS
24   HISHISHISHISHIS