BMRB Entry 18581

Name: Solution structure of gp78 CUE domain
Published: 2012-11-19

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PDB ID: 2lvn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18581
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of gp78 CUE domain

Deposition date:

2012-07-09

Original release date:

2012-11-19

Authors:

Liu, Shan; Chen, Yinghua; Huang, Tao; Tarasov, Sergey; King, Aaren; Li, Jess; Weissman, Allan; Byrd, Robert; Das, Ranabir

Citation:

Citation: Liu, Shan; Chen, Yinghua; Li, Jess; Huang, Tao; Tarasov, Sergey; King, Aaren; Weissman, Allan; Byrd, R. Andrew; Das, Ranabir. "Promiscuous interactions of gp78 E3 ligase CUE domain with polyubiquitin chains." Structure 20, 2138-2150 (2012).
PubMed: 23123110

Assembly members:

Assembly members:
entity, polymer, 52 residues, 5966.808 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: SNSQLNAMAHQIQEMFPQVP YHLVLQDLQLTRSVEITTDN ILEGRIQVPFPT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	231
15N chemical shifts	53
1H chemical shifts	378

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	gp78 CUE domain	1

Entities:

Entity 1, gp78 CUE domain 52 residues - 5966.808 Da.

1

SER

ASN

SER

GLN

LEU

ASN

ALA

MET

ALA

HIS

2

GLN

ILE

GLN

GLU

MET

PHE

PRO

GLN

VAL

PRO

3

TYR

HIS

LEU

VAL

LEU

GLN

ASP

LEU

GLN

LEU

4

THR

ARG

SER

VAL

GLU

ILE

THR

ASP

ASN

5

ILE

LEU

GLU

GLY

ARG

ILE

GLN

VAL

PRO

PHE

6

PRO

THR

Samples:

sample_1: TRIS 50 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker INOVA 600 MHz
Bruker INOVA 700 MHz
Varian INOVA 600 MHz
Varian INOVA 800 MHz

PDB	2EJS 2LVN 2LVO 2LVP 2LVQ 4G3O
DBJ	BAE01277 BAE34049 BAE41974 BAK63135
GB	AAD56721 AAD56722 AAH03256 AAH17043 AAH34538
REF	NP_001039439 NP_001135 NP_001267243 NP_035917 XP_001091030
SP	Q9R049 Q9UKV5
TPG	DAA20037
AlphaFold	Q9R049 Q9UKV5