BMRB Entry 18562

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18562
MolProbity Validation Chart

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Title:
Backbone and side-chain assignments of an effector membrane localization domain from Vibrio vulnificus MARTX toxin
Deposition date:
2012-06-30
Original release date:
2014-02-11
Authors:
Brothers, Michael; Geissler, Brett; Hsiao, Grant; Wilson, Brenda; Satchell, Karla; Rienstra, Chad
Citation:

Citation: Brothers, Michael; Geissler, Brett; Hisao, Grant; Wilson, Brenda; Satchell, Karla; Rienstra, Chad. "Backbone and side-chain assignments of an effector membrane localization domain from Vibrio vulnificus MARTX toxin."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 23765285

Assembly members:

Assembly members:
VvMARTX_MLD, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 672   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Vibrio Vulnificus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pYCpet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VvMARTX_MLD: MGQIFTVQELKERAKVFAKP IGASYQGILDQLDLVHQAKG RDQIAASFELNKKINDYIAE HPTSGRNQALTQLKEQVTSA LGLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts323
15N chemical shifts73
1H chemical shifts427

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1VvMARTX MLD1

Entities:

Entity 1, VvMARTX MLD 90 residues - Formula weight is not available

1   METGLYGLNILEPHETHRVALGLNGLULEU
2   LYSGLUARGALALYSVALPHEALALYSPRO
3   ILEGLYALASERTYRGLNGLYILELEUASP
4   GLNLEUASPLEUVALHISGLNALALYSGLY
5   ARGASPGLNILEALAALASERPHEGLULEU
6   ASNLYSLYSILEASNASPTYRILEALAGLU
7   HISPROTHRSERGLYARGASNGLNALALEU
8   THRGLNLEULYSGLUGLNVALTHRSERALA
9   LEUGLYLEUGLUHISHISHISHISHISHIS

Samples:

sample_1: DSS .01 mM; EDTA 2 mM; NaCl 500 mM; VvMARTX MLD, [U-99% 13C; U-99% 15N], 1 mM; Tris-HCl 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.5 M; pH: 7.4; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

VNMRJ v2.2, Varian - collection

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AIS39496 AIS39497 AIS39498 AIS39499 AIS39500

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks