BMRB Entry 18552

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18552

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Title:
TM4-Cx43CT
Deposition date:
2012-06-27
Original release date:
2013-02-20
Authors:
Grosely, Rosslyn; Kieken, Fabien; Sorgen, Paul
Citation:

Citation: Grosely, Rosslyn; Kieken, Fabien; Sorgen, Paul. "1H, 13C, and 15N backbone resonance assignments of the connexin43 carboxyl terminal domain attached to the 4th transmembrane domain in detergent micelles."  Biomol. NMR Assignments 7, 299-303 (2013).
PubMed: 23065337

Assembly members:

Assembly members:
TM4-Cx43CT, polymer, 207 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-14

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TM4-Cx43CT: MGSSHHHHHHSSGLVPRGSH MDCFLSRPTEKTIFIIFMLV VSLVSLALNIIELFYVFFKG VKDRVKGRSDPYHATTGPLS PSKDCGSPKYAYFNGCSSPT APLSPMSPPGYKLVTGDRNN SSCRNYNKQASEQNWANYSA EQNRMGQAGSTISNSHAQPF DFPDDNQNAKKVAAGHELQP LAIVDQRPSSRASSRASSRP RPDDLEI

Data sets:
Data typeCount
13C chemical shifts413
15N chemical shifts132
1H chemical shifts722

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TM4-Cx43CT monomer1

Entities:

Entity 1, TM4-Cx43CT monomer 207 residues - Formula weight is not available

The first 21 residues are the affinity tag and linker sequence. Asp 22 corresponds with Asp 197 of full-length Connexin43.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METASPCYSPHELEUSERARGPROTHRGLU
4   LYSTHRILEPHEILEILEPHEMETLEUVAL
5   VALSERLEUVALSERLEUALALEUASNILE
6   ILEGLULEUPHETYRVALPHEPHELYSGLY
7   VALLYSASPARGVALLYSGLYARGSERASP
8   PROTYRHISALATHRTHRGLYPROLEUSER
9   PROSERLYSASPCYSGLYSERPROLYSTYR
10   ALATYRPHEASNGLYCYSSERSERPROTHR
11   ALAPROLEUSERPROMETSERPROPROGLY
12   TYRLYSLEUVALTHRGLYASPARGASNASN
13   SERSERCYSARGASNTYRASNLYSGLNALA
14   SERGLUGLNASNTRPALAASNTYRSERALA
15   GLUGLNASNARGMETGLYGLNALAGLYSER
16   THRILESERASNSERHISALAGLNPROPHE
17   ASPPHEPROASPASPASNGLNASNALALYS
18   LYSVALALAALAGLYHISGLULEUGLNPRO
19   LEUALAILEVALASPGLNARGPROSERSER
20   ARGALASERSERARGALASERSERARGPRO
21   ARGPROASPASPLEUGLUILE

Samples:

sample_1: TM4-Cx43CT, [U-13C; U-15N], 0.850 mM; MES 20 mM; sodium chloride 50 mM; DTT 1 mM; EDTA 1 mM; LPPG 8%; TFE, [U-99% 2H], 10%

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAC26375 BAC29656 BAC38285 BAE26592 BAE38502
EMBL CAA29855 CAA43778 CAA44640 CAC21170 CAJ18535
GB AAA37444 AAH06894 AAH55375 AAH81842 AAM34255
PRF 1404339A 1714172A
REF NP_001002951 NP_001185877 NP_001231004 NP_001268545 NP_034418
SP P08050 P23242 Q6TYA7
AlphaFold P08050 P23242 Q6TYA7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks