BMRB Entry 18550

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PDB ID: 2lv1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18550
MolProbity Validation Chart

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Title:
Solution-state NMR of prion protein mutant V210I at pH 7
Deposition date:
2012-06-26
Original release date:
2012-09-17
Authors:
Biljan, Ivana; Ilc, Gregor; Giachin, Gabriele; Legname, Giuseppe; Plavec, Janez
Citation:

Citation: Biljan, Ivana; Ilc, Gregor; Giachin, Gabriele; Plavec, Janez; Legname, Giuseppe. "Structural Rearrangements at Physiological pH: Nuclear Magnetic Resonance Insights from the V210I Human Prion Protein Mutant."  Biochemistry 51, 7465-7474 (2012).
PubMed: 22947063

Assembly members:

Assembly members:
V210I, polymer, 147 residues, 16654.660 Da.

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pProExHTa

Entity Sequences (FASTA):

Entity Sequences (FASTA):
V210I: GAMDPGQGGGTHSQWNKPSK PKTNMKHMAGAAAAGAVVGG LGGYMLGSAMSRPIIHFGSD YEDRYYRENMHRYPNQVYYR PMDEYSNQNNFVHDCVNITI KQHTVTTTTKGENFTETDVK MMERVIEQMCITQYERESQA YYQRGSS

Data sets:
Data typeCount
13C chemical shifts460
15N chemical shifts132
1H chemical shifts915

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1V210I1

Entities:

Entity 1, V210I 147 residues - 16654.660 Da.

The five extra residues at the N-terminus are remnant of the tobacco etch virus (TEV) cleavege.

1   GLYALAMETASPPROGLYGLNGLYGLYGLY
2   THRHISSERGLNTRPASNLYSPROSERLYS
3   PROLYSTHRASNMETLYSHISMETALAGLY
4   ALAALAALAALAGLYALAVALVALGLYGLY
5   LEUGLYGLYTYRMETLEUGLYSERALAMET
6   SERARGPROILEILEHISPHEGLYSERASP
7   TYRGLUASPARGTYRTYRARGGLUASNMET
8   HISARGTYRPROASNGLNVALTYRTYRARG
9   PROMETASPGLUTYRSERASNGLNASNASN
10   PHEVALHISASPCYSVALASNILETHRILE
11   LYSGLNHISTHRVALTHRTHRTHRTHRLYS
12   GLYGLUASNPHETHRGLUTHRASPVALLYS
13   METMETGLUARGVALILEGLUGLNMETCYS
14   ILETHRGLNTYRGLUARGGLUSERGLNALA
15   TYRTYRGLNARGGLYSERSER

Related Database Links:

PDB 2lej 1E1G 1E1J 1E1P 1E1S 1E1U 1E1W 1FKC 1FO7 1HJM 1HJN 1I4M 1QLX 1QLZ 1QM0 1QM1 1QM2 1QM3 2K1D 2KUN 2LEJ 2LFT 2LSB 2LV1 2M8T 2W9E 3HAF 3HAK 3HEQ 3HER 3HES 3HJ5 3HJX 4DGI 4KML 4N9O
BMRB 15676 16743 16757 17714 17756 17757 17780 18426 19268 4379 4402 4434 4620 4641 4736
DBJ BAA00011 BAF62360 BAG32276 BAG32277 BAG32278
EMBL CAA58442 CAG46836 CAG46869
GB AAA19664 AAA60182 AAA68632 AAA68633 AAB59442
REF NP_000302 NP_001009093 NP_001073590 NP_001073591 NP_001073592
SP P04156 P40252 P61766 P61767 P61768
AlphaFold P04156 P40252 P61766 P61767 P61768

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