BMRB Entry 18541

Click here to enlarge.

PDB ID: 2luu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18541
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
NMR solution structure of midkine-b, mdkb
Deposition date:
2012-06-21
Original release date:
2013-04-02
Authors:
Yang, Daiwen; Lim, Jackwee; Meng, Dan
Citation:

Citation: Lim, Jackwee; Yao, Sheng; Graf, Martin; Winkler, Christoph; Yang, Daiwen. "Structure-function analysis of full-length midkine reveals novel residues important for heparin binding and zebrafish embryogenesis."  Biochem. J. 451, 407-415 (2013).
PubMed: 23418741

Assembly members:

Assembly members:
midkine-b, polymer, 127 residues, 14021.246 Da.

Natural source:

Natural source:   Common Name: Zebrafish   Taxonomy ID: 7955   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Danio rerio

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
midkine-b: GSMKKKEKGKEPKADAECSE WQYGKCVPNSGDCGNGIREA TCNEQTKKTKCKVPCNWKKD FGADCKYKFGRWAECDTTTG TRSRSGTLKKALFNAECQTT IKVSKPCTPKTPKPKGGEKK KGKGKEN

Data sets:
Data typeCount
13C chemical shifts331
15N chemical shifts115
1H chemical shifts565

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1midkine-b1

Entities:

Entity 1, midkine-b 127 residues - 14021.246 Da.

1   GLYSERMETLYSLYSLYSGLULYSGLYLYS
2   GLUPROLYSALAASPALAGLUCYSSERGLU
3   TRPGLNTYRGLYLYSCYSVALPROASNSER
4   GLYASPCYSGLYASNGLYILEARGGLUALA
5   THRCYSASNGLUGLNTHRLYSLYSTHRLYS
6   CYSLYSVALPROCYSASNTRPLYSLYSASP
7   PHEGLYALAASPCYSLYSTYRLYSPHEGLY
8   ARGTRPALAGLUCYSASPTHRTHRTHRGLY
9   THRARGSERARGSERGLYTHRLEULYSLYS
10   ALALEUPHEASNALAGLUCYSGLNTHRTHR
11   ILELYSVALSERLYSPROCYSTHRPROLYS
12   THRPROLYSPROLYSGLYGLYGLULYSLYS
13   LYSGLYLYSGLYLYSGLUASN

Related Database Links:

PDB 2LUU
GB AAD38156 AAG27035 AAH59575 ABC67288 AJG05944
REF NP_571791

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks