BMRB Entry 18522

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PDB ID: 2lui
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18522
MolProbity Validation Chart

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Title:
Chemical Shift Assignments for the PICK1 PDZ domain fused to the C10 DAT ligand
Deposition date:
2012-06-14
Original release date:
2013-06-17
Authors:
Erlendsson, Simon; Teilum, Kaare
Citation:

Citation: Erlendsson, Simon; Rathje, Mette; Heidarsson, Petur; Poulsen, Flemming; Madsen, Kenneth; Teilum, Kaare; Gether, Ulrik. "Protein interacting with C-kinase 1 (PICK1) binding promiscuity relies on unconventional PSD-95/discs-large/ZO-1 homology (PDZ) binding modes for nonclass II PDZ ligand"  J. Biol. Chem. 289, 25327-25340 (2014).
PubMed: 25023278

Assembly members:

Assembly members:
entity, polymer, 232 residues, 24838.865 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX4t2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GSPGIPVPGKVTLQKDAQNL IGISIGGGAQYCPCLYIVQV FDNTPAALDGTVAAGDEITG VNGRSIKGKTKVEVAKMIQE VKGEVTIHYNKLQADPKQLE VLFQGPQFTLRHWLKVGSPG IPVPGKVTLQKDAQNLIGIS IGGGAQYCPCLYIVQVFDNT PAALDGTVAAGDEITGVNGR SIKGKTKVEVAKMIQEVKGE VTIHYNKLQADPKQLEVLFQ GPQFTLRHWLKV

Data sets:
Data typeCount
13C chemical shifts382
15N chemical shifts106
1H chemical shifts638

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PICK1 PDZ domain fused to the C10 DAT ligand1

Entities:

Entity 1, PICK1 PDZ domain fused to the C10 DAT ligand 232 residues - 24838.865 Da.

1   GLYSERPROGLYILEPROVALPROGLYLYS
2   VALTHRLEUGLNLYSASPALAGLNASNLEU
3   ILEGLYILESERILEGLYGLYGLYALAGLN
4   TYRCYSPROCYSLEUTYRILEVALGLNVAL
5   PHEASPASNTHRPROALAALALEUASPGLY
6   THRVALALAALAGLYASPGLUILETHRGLY
7   VALASNGLYARGSERILELYSGLYLYSTHR
8   LYSVALGLUVALALALYSMETILEGLNGLU
9   VALLYSGLYGLUVALTHRILEHISTYRASN
10   LYSLEUGLNALAASPPROLYSGLNLEUGLU
11   VALLEUPHEGLNGLYPROGLNPHETHRLEU
12   ARGHISTRPLEULYSVALGLYSERPROGLY
13   ILEPROVALPROGLYLYSVALTHRLEUGLN
14   LYSASPALAGLNASNLEUILEGLYILESER
15   ILEGLYGLYGLYALAGLNTYRCYSPROCYS
16   LEUTYRILEVALGLNVALPHEASPASNTHR
17   PROALAALALEUASPGLYTHRVALALAALA
18   GLYASPGLUILETHRGLYVALASNGLYARG
19   SERILELYSGLYLYSTHRLYSVALGLUVAL
20   ALALYSMETILEGLNGLUVALLYSGLYGLU
21   VALTHRILEHISTYRASNLYSLEUGLNALA
22   ASPPROLYSGLNLEUGLUVALLEUPHEGLN
23   GLYPROGLNPHETHRLEUARGHISTRPLEU
24   LYSVAL

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