BMRB Entry 18518
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PDB ID: 2lue
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18518
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Rogov, Vladimir; Suzuki, Hironori; Fiskin, Evgenij; Wild, Philipp; Kniss, Andreas; Rozenknop, Alexis; Kato, Ryuichi; Kawasaki, Masato; McEwan, David; Lohr, Frank; Guntert, Peter; Dikic, Ivan; Wakatsuki, Soichi; Dotsch, Volker. "Structural basis for phosphorylation-triggered autophagic clearance of Salmonella." Biochem. J. 454, 459-466 (2013).
PubMed: 23805866
Assembly members:
entity_1, polymer, 119 residues, 13965.232 Da.
entity_2, polymer, 17 residues, 2245.827 Da.
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETm60_UB3
Entity Sequences (FASTA):
entity_1: GAMGKTFKQRRTFEQRVEDV
RLIREQHPTKIPVIIERYKG
EKQLPVLDKTKFLVPDHVNM
SELIKIIRRRLQLNANQAFF
LLVNGHSMVSVSTPISEVYE
SEKDEDGFLYMVYASQETF
entity_2: NXXGXXEDXFVEIRMAE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 548 |
| 15N chemical shifts | 141 |
| 1H chemical shifts | 1028 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 119 residues - 13965.232 Da.
Residues 1-4 are expression tag. Residues 5-119 correspond to residues 5-119 of human Microtubule-associated proteins 1A/1B light chain 3B (UniProtKB entry Q9GZQ8)
| 1 | GLY | ALA | MET | GLY | LYS | THR | PHE | LYS | GLN | ARG | ||||
| 2 | ARG | THR | PHE | GLU | GLN | ARG | VAL | GLU | ASP | VAL | ||||
| 3 | ARG | LEU | ILE | ARG | GLU | GLN | HIS | PRO | THR | LYS | ||||
| 4 | ILE | PRO | VAL | ILE | ILE | GLU | ARG | TYR | LYS | GLY | ||||
| 5 | GLU | LYS | GLN | LEU | PRO | VAL | LEU | ASP | LYS | THR | ||||
| 6 | LYS | PHE | LEU | VAL | PRO | ASP | HIS | VAL | ASN | MET | ||||
| 7 | SER | GLU | LEU | ILE | LYS | ILE | ILE | ARG | ARG | ARG | ||||
| 8 | LEU | GLN | LEU | ASN | ALA | ASN | GLN | ALA | PHE | PHE | ||||
| 9 | LEU | LEU | VAL | ASN | GLY | HIS | SER | MET | VAL | SER | ||||
| 10 | VAL | SER | THR | PRO | ILE | SER | GLU | VAL | TYR | GLU | ||||
| 11 | SER | GLU | LYS | ASP | GLU | ASP | GLY | PHE | LEU | TYR | ||||
| 12 | MET | VAL | TYR | ALA | SER | GLN | GLU | THR | PHE |
Entity 2, entity_2 17 residues - 2245.827 Da.
Residues 169-185 correspond to residues 169-185 of human Optineurin sequence (UniProtKB entry Q96CV9). All serines in the peptide are phosphoserines (SEP).
| 1 | ASN | SEP | SEP | GLY | SEP | SEP | GLU | ASP | SEP | PHE | ||||
| 2 | VAL | GLU | ILE | ARG | MET | ALA | GLU |
Related Database Links:
| UNP | Q9GZQ8 Q96CV9 |
| BMRB | 15877 |
| PDB | 1UGM 1V49 2K6Q 2LUE 2Z0D 2Z0E 2ZJD 2ZZP 3VTU 3VTV 3VTW 3WAO 3X0W 4WAA 5D94 |
| DBJ | BAB15169 BAB22364 BAB22569 BAB22641 BAB22855 |
| EMBL | CAD38970 CAG31435 CAL38438 |
| GB | AAA20645 AAB72082 AAG09686 AAG23182 AAH18634 |
| REF | NP_001001169 NP_001026632 NP_001078950 NP_001177219 NP_001180554 |
| SP | A6NCE7 O41515 Q62625 Q9CQV6 Q9GZQ8 |
| TPG | DAA20021 |
| AlphaFold | Q6NW02 A6NCE7 O41515 Q62625 Q9CQV6 Q9GZQ8 Q9Y218 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks