BMRB Entry 18516

Title:
1H, 13C and 15N backbone NMR reassignment of the third PDZ domain of PSD95 protein
Deposition date:
2012-06-12
Original release date:
2014-04-22
Authors:
Candel, Adela M.; Murciano-Calles, Javier; Martinez, Jose C.
Citation:

Citation: Murciano-Calles, Javier; Corbi-Verge, Carles; Candel, Adela; Luque, Irene; Martinez, Jose. "Post-translational modifications modulate ligand recognition by the third PDZ domain of the MAGUK protein PSD-95."  PLoS ONE 9, e90030-e90030 (2014).
PubMed: 24587199

Assembly members:

Assembly members:
PDZ3, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBAT4

Data sets:
Data typeCount
13C chemical shifts284
15N chemical shifts101
1H chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ31

Entities:

Entity 1, PDZ3 103 residues - Formula weight is not available

1   GLYLEUGLYGLUGLUASPILEPROARGGLU
2   PROARGARGILEVALILEHISARGGLYSER
3   THRGLYLEUGLYPHEASNILEVALGLYGLY
4   GLUASPGLYGLUGLYILEPHEILESERPHE
5   ILELEUALAGLYGLYPROALAASPLEUSER
6   GLYGLULEUARGLYSGLYASPGLNILELEU
7   SERVALASNGLYVALASPLEUARGASNALA
8   SERHISGLUGLNALAALAILEALALEULYS
9   ASNALAGLYGLNTHRVALTHRILEILEALA
10   GLNTYRLYSPROGLUGLUTYRSERARGPHE
11   GLUALALYS

Samples:

sample_1: PDZ3, [U-95% 13C; U-95% 15N], 1 mM; H2O, [U-95% 13C; U-95% 15N], 90%; D2O, [U-95% 13C; U-95% 15N], 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
REF XP_005669278 XP_008122573 XP_012511057 XP_012868584

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks