BMRB Entry 18480
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PDB ID: 2lth
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18480
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Kronqvist, Nina; Otikovs, Martins; Chmyrov, Volodymyr; Chen, Gefei; Andersson, Marlene; Nordling, Kerstin; Landreh, Michael; Sarr, Medoune; Jornvall, Hans; Wennmalm, Stefan; Widengren, Jerker; Meng, Qing; Rising, Anna; Otzen, Daniel; Knight, Stefan; Jaudzems, Kristaps; Johansson, Jan. "Sequential pH-driven dimerization and stabilization of the N-terminal domain enables rapid spider silk formation." Nat. Commun. 5, 3254-3254 (2014).
PubMed: 24510122
Assembly members:
Major_ampullate_spidroin_1, polymer, 137 residues, 14183.802 Da.
Natural source: Common Name: spider Taxonomy ID: 332052 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Euprosthenops australis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: not applicable
Entity Sequences (FASTA):
Major_ampullate_spidroin_1: GSGNSHTTPWTNPGLAENFM
NSFMQGLSSMPGFTASQLDD
MSTIAQSMVQSIQSLAAQGR
TSPNKLQALNMAFASSMAEI
AASEEGGGSLSTKTSSIASA
MSNAFLQTTGVVNQPFINEI
TQLVSMFAQAGMNDVSA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 537 |
| 15N chemical shifts | 152 |
| 1H chemical shifts | 893 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 1 |
Entities:
Entity 1, entity_1 137 residues - 14183.802 Da.
| 1 | GLY | SER | GLY | ASN | SER | HIS | THR | THR | PRO | TRP | ||||
| 2 | THR | ASN | PRO | GLY | LEU | ALA | GLU | ASN | PHE | MET | ||||
| 3 | ASN | SER | PHE | MET | GLN | GLY | LEU | SER | SER | MET | ||||
| 4 | PRO | GLY | PHE | THR | ALA | SER | GLN | LEU | ASP | ASP | ||||
| 5 | MET | SER | THR | ILE | ALA | GLN | SER | MET | VAL | GLN | ||||
| 6 | SER | ILE | GLN | SER | LEU | ALA | ALA | GLN | GLY | ARG | ||||
| 7 | THR | SER | PRO | ASN | LYS | LEU | GLN | ALA | LEU | ASN | ||||
| 8 | MET | ALA | PHE | ALA | SER | SER | MET | ALA | GLU | ILE | ||||
| 9 | ALA | ALA | SER | GLU | GLU | GLY | GLY | GLY | SER | LEU | ||||
| 10 | SER | THR | LYS | THR | SER | SER | ILE | ALA | SER | ALA | ||||
| 11 | MET | SER | ASN | ALA | PHE | LEU | GLN | THR | THR | GLY | ||||
| 12 | VAL | VAL | ASN | GLN | PRO | PHE | ILE | ASN | GLU | ILE | ||||
| 13 | THR | GLN | LEU | VAL | SER | MET | PHE | ALA | GLN | ALA | ||||
| 14 | GLY | MET | ASN | ASP | VAL | SER | ALA |
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