BMRB Entry 18474

Name: the pwwp domain of TFIIS2-1 from Trypanosoma brucei
Published: 2012-08-29

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PDB ID: 2m1h
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18474
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

the pwwp domain of TFIIS2-1 from Trypanosoma brucei

Deposition date:

2012-05-18

Original release date:

2012-08-29

Authors:

Wang, Rui; Fan, Kai; Liao, Shanhui; Zhang, Jiahai; Tu, Xiaoming

Citation:

Citation: Zhang, Jiahai; Dai, Kun; Liao, Shanhui; Tu, Xiaoming. "H, C and N resonance assignments of TbTFIIS2-2 PWWP domain from Trypanosoma brucei." Biomol. NMR Assignments 7, 207-209 (2013).
PubMed: 22836947

Assembly members:

Assembly members:
entity, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MLQERVFHINDRVWLKTGAN TWWPAKVTSVTGVEGVDGRS SETGTSTVTVLTYPGTQNKA TYKNVDSHSSAITFFEPSSE KAVTANEDLLQAIRNAEEDK ESNALRFEPTLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	294
15N chemical shifts	107
1H chemical shifts	614

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	pwwp domain of TFIIS2-1	1

Entities:

Entity 1, pwwp domain of TFIIS2-1 118 residues - Formula weight is not available

1

MET

LEU

GLN

GLU

ARG

VAL

PHE

HIS

ILE

ASN

2

ASP

ARG

VAL

TRP

LEU

LYS

THR

GLY

ALA

ASN

3

THR

TRP

PRO

ALA

LYS

VAL

THR

SER

VAL

4

THR

GLY

VAL

GLU

GLY

VAL

ASP

GLY

ARG

SER

5

SER

GLU

THR

GLY

THR

SER

THR

VAL

THR

VAL

6

LEU

THR

TYR

PRO

GLY

THR

GLN

ASN

LYS

ALA

7

THR

TYR

LYS

ASN

VAL

ASP

SER

HIS

SER

8

ALA

ILE

THR

PHE

GLU

PRO

SER

GLU

9

LYS

ALA

VAL

THR

ALA

ASN

GLU

ASP

LEU

10

GLN

ALA

ILE

ARG

ASN

ALA

GLU

ASP

LYS

11

GLU

SER

ASN

ALA

LEU

ARG

PHE

GLU

PRO

THR

12

LEU

GLU

HIS

Samples:

sample_1: Tbpwwp, [U-100% 13C; U-100% 15N], 0.5 mM; H2O 90%; D2O 10%; Na2HPO4 25 mM; NaCl 150 mM; EDTA 2 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.7; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker DMX 500 MHz

PDB	2M1H
EMBL	CBH09458
GB	AAQ15815 AAX79612
REF	XP_011771763 XP_951597