BMRB Entry 18434

Name: C-terminal domain of human REV1 in complex with DNA-polymerase H (eta)
Published: 2012-06-18

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PDB ID: 2lsk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18434
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

C-terminal domain of human REV1 in complex with DNA-polymerase H (eta)

Deposition date:

2012-05-01

Original release date:

2012-06-18

Authors:

Pozhidaeva, Alexandra; Pustovalova, Yulia; Pustovalova, Irina; Korzhnev, Dmitry

Citation:

Citation: Pozhidaeva, Alexandra; Pustovalova, Yulia; Bezsonova, Sanjay; Walker, Irina; Korzhnev, Graham. "NMR structure and dynamics of the C-terminal domain from human Rev1 and its complex with Rev1 interacting region of DNA polymerase ." Biochemistry 51, 5506-5520 (2012).
PubMed: 22691049

Assembly members:

Assembly members:
entity_1, polymer, 95 residues, 11011.758 Da.
entity_2, polymer, 16 residues, 1826.117 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GNLAGAVEFNDVKTLLREWI TTISDPMEEDILQVVKYCTD LIEEKDLEKLDLVIKYMKRL MQQSVESVWNMAFDFILDNV QVVLQQTYGSTLKVT
entity_2: QSTGTEPFFKQKSLLL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	382
15N chemical shifts	97
1H chemical shifts	639

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-terminal domain of human REV1	1
2	DNA-polymerase H (eta)	2

Entities:

Entity 1, C-terminal domain of human REV1 95 residues - 11011.758 Da.

1

GLY

ASN

LEU

ALA

GLY

ALA

VAL

GLU

PHE

ASN

2

ASP

VAL

LYS

THR

LEU

ARG

GLU

TRP

ILE

3

THR

ILE

SER

ASP

PRO

MET

GLU

ASP

4

ILE

LEU

GLN

VAL

LYS

TYR

CYS

THR

ASP

5

LEU

ILE

GLU

LYS

ASP

LEU

GLU

LYS

LEU

6

ASP

LEU

VAL

ILE

LYS

TYR

MET

LYS

ARG

LEU

7

MET

GLN

SER

VAL

GLU

SER

VAL

TRP

ASN

8

MET

ALA

PHE

ASP

PHE

ILE

LEU

ASP

ASN

VAL

9

GLN

VAL

LEU

GLN

THR

TYR

GLY

SER

10

THR

LEU

LYS

VAL

THR

Entity 2, DNA-polymerase H (eta) 16 residues - 1826.117 Da.

1

GLN

SER

THR

GLY

THR

GLU

PRO

PHE

LYS

2

GLN

LYS

SER

LEU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

BMRB	18432 18455
PDB	2LSI 2LSK 2LSY 3VU7 4BA9 4EXT 4GK0 4GK5 2LSK
DBJ	BAB21441 BAA81666 BAB18601 BAG51237 BAG53738 BAG57675
EMBL	CAB38231 CAH93279
GB	AAF06731 AAF18986 AAI30412 AAK43708 AAY24314 AAD43810 AAQ81300 ACQ91143 EAX04210 EAX04211
REF	NP_001032961 NP_001126930 NP_057400 XP_001160264 XP_001363717 NP_001278898 NP_006493 XP_003833292 XP_004048392 XP_005249243
SP	Q5R4N7 Q9UBZ9 Q9Y253
AlphaFold	Q5R4N7 Q9UBZ9 Q9Y253

BMRB Entry 18434

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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