BMRB Entry 18417

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18417

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Title:
1H, 13C and 15N resonance assignments of human BASP1
Deposition date:
2012-04-24
Original release date:
2012-12-13
Authors:
Geist, Leonhard; Zawadzka-Kazimierczuk, Anna; Saxena, Saurabh; Zerko, Szymon; Kozminski, Wiktor; Konrat, Robert
Citation:

Citation: Geist, Leonhard; Zawadzka-Kazimierczuk, Anna; Saxena, Saurabh; erko, Szymon; Komiski, Wiktor; Konrat, Robert. "1H, 13C and 15N resonance assignments of human BASP1."  Biomol. NMR Assignments 7, 315-319 (2013).
PubMed: 23179057

Assembly members:

Assembly members:
H6-hBASP1, polymer, 253 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-M11-hBASP1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H6-hBASP1: MKHHHHHHPMSDYDIPTTEN LYFQGAMGGKLSKKKKGYNV NDEKAKEKDKKAEGAATEEE GTPKESEPQAAAEPAEAKEG KEKPDQDAEGKAEEKEGEKD AAAAKEEAPKAEPEKTEGAA EAKAEPPKAPEQEQAAPGPA AGGEAPKAAEAAAAPAESAA PAAGEEPSKEEGEPKKTEAP AAPAAQETKSDGAPASDSKP GSSEAAPSSKETPAATEAPS STPKAQGPAASAEEPKPVEA PAANSDQTVTVKE

Data sets:
Data typeCount
13C chemical shifts798
15N chemical shifts240
1H chemical shifts1089

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H6-hBASP11

Entities:

Entity 1, H6-hBASP1 253 residues - Formula weight is not available

1   METLYSHISHISHISHISHISHISPROMET
2   SERASPTYRASPILEPROTHRTHRGLUASN
3   LEUTYRPHEGLNGLYALAMETGLYGLYLYS
4   LEUSERLYSLYSLYSLYSGLYTYRASNVAL
5   ASNASPGLULYSALALYSGLULYSASPLYS
6   LYSALAGLUGLYALAALATHRGLUGLUGLU
7   GLYTHRPROLYSGLUSERGLUPROGLNALA
8   ALAALAGLUPROALAGLUALALYSGLUGLY
9   LYSGLULYSPROASPGLNASPALAGLUGLY
10   LYSALAGLUGLULYSGLUGLYGLULYSASP
11   ALAALAALAALALYSGLUGLUALAPROLYS
12   ALAGLUPROGLULYSTHRGLUGLYALAALA
13   GLUALALYSALAGLUPROPROLYSALAPRO
14   GLUGLNGLUGLNALAALAPROGLYPROALA
15   ALAGLYGLYGLUALAPROLYSALAALAGLU
16   ALAALAALAALAPROALAGLUSERALAALA
17   PROALAALAGLYGLUGLUPROSERLYSGLU
18   GLUGLYGLUPROLYSLYSTHRGLUALAPRO
19   ALAALAPROALAALAGLNGLUTHRLYSSER
20   ASPGLYALAPROALASERASPSERLYSPRO
21   GLYSERSERGLUALAALAPROSERSERLYS
22   GLUTHRPROALAALATHRGLUALAPROSER
23   SERTHRPROLYSALAGLNGLYPROALAALA
24   SERALAGLUGLUPROLYSPROVALGLUALA
25   PROALAALAASNSERASPGLNTHRVALTHR
26   VALLYSGLU

Samples:

sample_1: H6-hBASP1, [U-95% 13C; U-90% 15N], 2 mM; Bis-Tris 20 mM; sodium chloride 50 mM; sodium azide 0.2%; D2O, [U-100% 2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 50 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
5D HN(CA)CONHsample_1isotropicsample_conditions_1
5D (HACA)CON(CA)CONHsample_1isotropicsample_conditions_1
5D (H)NCO(NCA)CONHsample_1isotropicsample_conditions_1
5D HNCOCACBsample_1isotropicsample_conditions_1
5D HabCabCONHsample_1isotropicsample_conditions_1
5D H(CC-tocsy)CONHsample_1isotropicsample_conditions_1

Software:

MFT, Wiktor Kozminski - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Agilent Direct Drive 700 MHz

Related Database Links:

UNP P80723
GB AAC67374 AAH00518 AAV38147 AAV38148 AAX41438
REF NP_001258535 NP_006308 XP_001087717 XP_003263198 XP_003822013
SP P80723
AlphaFold Q9BWA5 P80723

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks