BMRB Entry 18416

Name: 1H,15N and 13C backbone and side chain chemical shifts of the ubiquitin homology domain of mouse BAG-1
Published: 2012-09-14

Click here to enlarge.

PDB ID: 2lwp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18416
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H,15N and 13C backbone and side chain chemical shifts of the ubiquitin homology domain of mouse BAG-1

Deposition date:

2012-04-24

Original release date:

2012-09-14

Authors:

Huang, Hsiao Wen; Yu, Chin

Citation:

Citation: Huang, Hsiao-Wen; Yu, Chin. "Backbone and side-chain resonance assignments (1H, 15N and 13C) of the ubiquitin homology domain of mouse BAG-1." Biomol. NMR Assignments 7, 235-239 (2013).
PubMed: 22903788

Assembly members:

Assembly members:
ubiquitin_homology_domain_of_mouse_BAG-1, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ubiquitin_homology_domain_of_mouse_BAG-1: MAKTEEMVQTEEMETPRLSV IVTHSNERYDLLVTPQQGNS EPVVQDLAQLVEEATGVPLP FQKLIFKGKSLKEMETPLSA LGMQNGCRVMLIGEKSN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	383
15N chemical shifts	96
1H chemical shifts	634

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ubiquitin homology of mouse BAG-1	1

Entities:

Entity 1, ubiquitin homology of mouse BAG-1 97 residues - Formula weight is not available

1

MET

ALA

LYS

THR

GLU

MET

VAL

GLN

THR

2

GLU

MET

GLU

THR

PRO

ARG

LEU

SER

VAL

3

ILE

VAL

THR

HIS

SER

ASN

GLU

ARG

TYR

ASP

4

LEU

VAL

THR

PRO

GLN

GLY

ASN

SER

5

GLU

PRO

VAL

GLN

ASP

LEU

ALA

GLN

LEU

6

VAL

GLU

ALA

THR

GLY

VAL

PRO

LEU

PRO

7

PHE

GLN

LYS

LEU

ILE

PHE

LYS

GLY

LYS

SER

8

LEU

LYS

GLU

MET

GLU

THR

PRO

LEU

SER

ALA

9

LEU

GLY

MET

GLN

ASN

GLY

CYS

ARG

VAL

MET

10

LEU

ILE

GLY

GLU

LYS

SER

ASN

Samples:

sample_1: ubiquitin homology domain of mouse BAG-1, [U-100% 13C; U-100% 15N], 1.0 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1

Software:

VNMR, Varian - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

Varian VNMR 700 MHz

PDB	2LWP 2M8S
DBJ	BAB26106
GB	AAC34259 AAH03722 AAH69918 AAH93509 EDL05422
REF	NP_001165210 NP_033866
SP	Q60739
AlphaFold	Q60739 Q60739