BMRB Entry 18395

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18395

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Title:
Chemical shift assignments of deuterated DsbB by 1H-detected solid-state NMR
Deposition date:
2012-04-13
Original release date:
2012-09-24
Authors:
Zhou, Donghua; Nieuwkoop, Andrew; Berthold, Deborah; Comellas, Gemma; Sperling, Lindsay; Tang, Ming; Shah, Gautam; Brea, Elliot; Lemkau, Luisel; Rienstra, Chad
Citation:

Citation: Zhou, Donghua; Nieuwkoop, Andrew; Berthold, Deborah; Comellas, Gemma; Sperling, Lindsay; Tang, Ming; Shah, Gautam; Brea, Elliott; Lemkau, Luisel; Rienstra, Chad. "Solid-state NMR analysis of membrane proteins and protein aggregates by proton detected spectroscopy."  J. Biomol. NMR 54, 291-305 (2012).
PubMed: 22986689

Assembly members:

Assembly members:
DsbB, polymer, 176 residues, 15287.664 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE70

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DsbB: MLRFLNQASQGRGAWLLMAF TALALELTALWFQHVMLLKP CVLCIYERVALFGVLGAALI GAIAPKTPLRYVAMVIWLYS AFRGVQLTYEHTMLQLYPSP FATCDFMVRFPEWLPLDKWV PQVFVASGDCAERQWDFLGL EMPQWLLGIFIAYLIVAVLV VISQPFKAKKRDLFGR

Data sets:
Data typeCount
13C chemical shifts132
15N chemical shifts67
1H chemical shifts67

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1DsbB1

Entities:

Entity 1, DsbB 176 residues - 15287.664 Da.

1   METLEUARGPHELEUASNGLNALASERGLN
2   GLYARGGLYALATRPLEULEUMETALAPHE
3   THRALALEUALALEUGLULEUTHRALALEU
4   TRPPHEGLNHISVALMETLEULEULYSPRO
5   CYSVALLEUCYSILETYRGLUARGVALALA
6   LEUPHEGLYVALLEUGLYALAALALEUILE
7   GLYALAILEALAPROLYSTHRPROLEUARG
8   TYRVALALAMETVALILETRPLEUTYRSER
9   ALAPHEARGGLYVALGLNLEUTHRTYRGLU
10   HISTHRMETLEUGLNLEUTYRPROSERPRO
11   PHEALATHRCYSASPPHEMETVALARGPHE
12   PROGLUTRPLEUPROLEUASPLYSTRPVAL
13   PROGLNVALPHEVALALASERGLYASPCYS
14   ALAGLUARGGLNTRPASPPHELEUGLYLEU
15   GLUMETPROGLNTRPLEULEUGLYILEPHE
16   ILEALATYRLEUILEVALALAVALLEUVAL
17   VALILESERGLNPROPHELYSALALYSLYS
18   ARGASPLEUPHEGLYARG

Related Database Links:

BMRB 15546 15966 18493
PDB 2HI7 2K73 2K74 2LEG 2LTQ 2ZUP 2ZUQ 3E9J
DBJ BAA07408 BAA36032 BAB35103 BAG76757 BAI24997
EMBL CAP75720 CAQ31687 CAQ98064 CAR02574 CAR07527
GB AAA23711 AAB25233 AAC74269 AAG56036 AAN42789
PIR H85696
REF NP_309707 NP_415703 NP_707082 WP_000652474 WP_000943441
SP A1AAA8 P0A6M2 P0A6M3 P59343 Q0T5L6
AlphaFold A1AAA8 P0A6M2 P0A6M3 P59343 Q0T5L6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks