BMRB Entry 18380

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18380

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments and Complete 15N Relaxation Analysis of the Soluble C-terminal Domain of CcmE Heme Chaperone from Desulfovibrio vulgaris, dvCcmE(44-137). Northeast Structural Genomics Target DvR115.
Deposition date:
2012-04-06
Original release date:
2012-04-26
Authors:
Aramini, James; Hamilton, Keith; Rossi, Paolo; Wang, Huang; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano
Citation:

Citation: Aramini, James; Hamilton, Keith; Rossi, Paolo; Ertekin, Asli; Lee, Hsiau-Wei; Lemak, Alexander; Wang, Huang; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano. "Solution NMR structure, backbone dynamics, and heme-binding properties of a novel cytochrome c maturation protein CcmE from Desulfovibrio vulgaris."  Biochemistry 51, 3705-3707 (2012).
PubMed: 22497251

Assembly members:

Assembly members:
dvCcmE', polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: d-proteobacteria   Taxonomy ID: 881   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Desulfovibrio vulgaris

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15TEV_NESG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dvCcmE': SHMATPQDKLHTVRLFGTVA ADGLTMLDGAPGVRFRLEDK DNTSKTVWVLYKGAVPDTFK PGVEVIIEGGLAPGEDTFKA RTLMTKCPSKYQKENRG

Data sets:
Data typeCount
13C chemical shifts270
15N chemical shifts93
1H chemical shifts304
heteronuclear NOE values82
T1 relaxation values82
T2 relaxation values82

Time Domain Data

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1dvCcmE'1

Entities:

Entity 1, dvCcmE' 97 residues - Formula weight is not available

N-terminal SHM remain after TEV cleavage

1   SERHISMETALATHRPROGLNASPLYSLEU
2   HISTHRVALARGLEUPHEGLYTHRVALALA
3   ALAASPGLYLEUTHRMETLEUASPGLYALA
4   PROGLYVALARGPHEARGLEUGLUASPLYS
5   ASPASNTHRSERLYSTHRVALTRPVALLEU
6   TYRLYSGLYALAVALPROASPTHRPHELYS
7   PROGLYVALGLUVALILEILEGLUGLYGLY
8   LEUALAPROGLYGLUASPTHRPHELYSALA
9   ARGTHRLEUMETTHRLYSCYSPROSERLYS
10   TYRGLNLYSGLUASNARGGLY

Related Database Links:

GB AAS95531 ABM28958 ADP86134
REF WP_010938350 WP_011792563 YP_010272

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks