BMRB Entry 18351

Name: Solution structure of the Class II hydrophobin NC2
Published: 2013-06-04

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PDB ID: 4aog
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18351
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Class II hydrophobin NC2

Deposition date:

2012-03-27

Original release date:

2013-06-04

Authors:

Ren, Qin; Kwan, Ann; Sunde, Margaret

Citation:

Citation: Ren, Qin; Kwan, Ann; Sunde, Margaret. "Solution structure and interface-driven self-assembly of NC2, a new member of the Class II hydrophobin proteins." Proteins ., .-. (2013).
PubMed: 24218020

Assembly members:

Assembly members:
nc2, polymer, 80 residues, 8187.3024 Da.

Natural source:

Natural source: Common Name: Neurospora crassa Taxonomy ID: 5141 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Neurospora crassa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHUE

Entity Sequences (FASTA):

Entity Sequences (FASTA):
nc2: SPAAMERQVPYTPCSGLYGT AQCCATDVLGVADLDCANPP ATLANATHFESTCAAIGQRA RCCVLPILGQDILCQTPAGL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list
- assigned_chem_shift_list_2
heteronucl_NOEs
- heteronuclear_noe_list
heteronucl_T1_relaxation
- heteronuclear_T1_list
heteronucl_T2_relaxation
- heteronuclear_T2_list
spectral_peak_list

Data type	Count
13C chemical shifts	327
15N chemical shifts	88
1H chemical shifts	536
T1 relaxation values	71
T2 relaxation values	71
heteronuclear NOE values	71

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	nc2	1

Entities:

Entity 1, nc2 80 residues - 8187.3024 Da.

1	SER	PRO	ALA	ALA	MET	GLU	ARG	GLN	VAL	PRO
2	TYR	THR	PRO	CYS	SER	GLY	LEU	TYR	GLY	THR
3	ALA	GLN	CYS	CYS	ALA	THR	ASP	VAL	LEU	GLY
4	VAL	ALA	ASP	LEU	ASP	CYS	ALA	ASN	PRO	PRO
5	ALA	THR	LEU	ALA	ASN	ALA	THR	HIS	PHE	GLU
6	SER	THR	CYS	ALA	ALA	ILE	GLY	GLN	ARG	ALA
7	ARG	CYS	CYS	VAL	LEU	PRO	ILE	LEU	GLY	GLN
8	ASP	ILE	LEU	CYS	GLN	THR	PRO	ALA	GLY	LEU

Name	Sample	Sample state	Sample conditions
T2:1 (H[n[T2(N)]])	13C15N_03	isotropic	T2-1
T2:2 (H[n[T2(N)]])	13C15N_03	isotropic	T2-2
T2:3 (H[n[T2(N)]])	13C15N_03	isotropic	T2-3
T2:4 (H[n[T2(N)]])	13C15N_03	isotropic	T2-4
T2:5 (H[n[T2(N)]])	13C15N_03	isotropic	T2-5
T2:6 (H[n[T2(N)]])	13C15N_03	isotropic	T2-6
T2:7 (H[n[T2(N)]])	13C15N_03	isotropic	T2-7
T2:8 (H[n[T2(N)]])	13C15N_03	isotropic	T2-8
T2:9 (H[n[T2(N)]])	13C15N_03	isotropic	T2-9
T2:10 (H[n[T2(N)]])	13C15N_03	isotropic	T2-10
T2:11 (H[n[T2(N)]])	13C15N_03	isotropic	T2-11
T2:12 (H[n[T2(N)]])	13C15N_03	isotropic	T2-12
T2:13 (H[n[T2(N)]])	13C15N_03	isotropic	T2-13
T2:14 (H[n[T2(N)]])	13C15N_03	isotropic	T2-14
T2:15 (H[n[T2(N)]])	13C15N_03	isotropic	T2-15
T1:1 (H[n[T1(N)]])	13C15N_03	isotropic	T1-1
T1:2 (H[n[T1(N)]])	13C15N_03	isotropic	T1-2
T1:3 (H[n[T1(N)]])	13C15N_03	isotropic	T1-3
T1:4 (H[n[T1(N)]])	13C15N_03	isotropic	T1-4
T1:5 (H[n[T1(N)]])	13C15N_03	isotropic	T1-5
T1:6 (H[n[T1(N)]])	13C15N_03	isotropic	T1-6
T1:7 (H[n[T1(N)]])	13C15N_03	isotropic	T1-7
T1:8 (H[n[T1(N)]])	13C15N_03	isotropic	T1-8
T1:9 (H[n[T1(N)]])	13C15N_03	isotropic	T1-9
T1:10 (H[n[T1(N)]])	13C15N_03	isotropic	T1-10
T1:11 (H[n[T1(N)]])	13C15N_03	isotropic	T1-11
T1:12 (H[n[T1(N)]])	13C15N_03	isotropic	T1-12
htNOE 1 (H[n[Trho(N)]])	13C15N_03	isotropic	CondSet1
htNOE 2 (H[n[Trho(N)]])	13C15N_03	isotropic	CondSet1
3D 1H-13C NOESY	13C15N_02	isotropic	CondSet1
3D 1H-13C NOESY	13C15N_02	isotropic	CondSet1
3D 1H-15N NOESY	15N	isotropic	CondSet1
2D 1H-1H NOESY	unlabeled	isotropic	CondSet1
3D HNCO	13C15N_01	isotropic	CondSet1
HNcaCO (H[N[ca[CO]]])	13C15N_01	isotropic	CondSet1
T2	sample_new_1	solution	CondSet1
T1	sample_new_1	solution	CondSet1
HTNOE 1	sample_new_1	solution	CondSet1

GenBank	TmpAcc
UNP	Q7S3P5_NEUCR
PDB	4AOG
GB	EAA30046 KHE86439
REF	XP_959282
AlphaFold	Q7S3P5

BMRB Entry 18351

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	SER	PRO	ALA	ALA	MET	GLU	ARG	GLN	VAL	PRO
2	TYR	THR	PRO	CYS	SER	GLY	LEU	TYR	GLY	THR
3	ALA	GLN	CYS	CYS	ALA	THR	ASP	VAL	LEU	GLY
4	VAL	ALA	ASP	LEU	ASP	CYS	ALA	ASN	PRO	PRO
5	ALA	THR	LEU	ALA	ASN	ALA	THR	HIS	PHE	GLU
6	SER	THR	CYS	ALA	ALA	ILE	GLY	GLN	ARG	ALA
7	ARG	CYS	CYS	VAL	LEU	PRO	ILE	LEU	GLY	GLN
8	ASP	ILE	LEU	CYS	GLN	THR	PRO	ALA	GLY	LEU

1	SER	PRO	ALA	ALA	MET	GLU	ARG	GLN	VAL	PRO
2	TYR	THR	PRO	CYS	SER	GLY	LEU	TYR	GLY	THR
3	ALA	GLN	CYS	CYS	ALA	THR	ASP	VAL	LEU	GLY
4	VAL	ALA	ASP	LEU	ASP	CYS	ALA	ASN	PRO	PRO
5	ALA	THR	LEU	ALA	ASN	ALA	THR	HIS	PHE	GLU
6	SER	THR	CYS	ALA	ALA	ILE	GLY	GLN	ARG	ALA
7	ARG	CYS	CYS	VAL	LEU	PRO	ILE	LEU	GLY	GLN
8	ASP	ILE	LEU	CYS	GLN	THR	PRO	ALA	GLY	LEU

1	SER	PRO	ALA	ALA	MET	GLU	ARG	GLN	VAL	PRO
2	TYR	THR	PRO	CYS	SER	GLY	LEU	TYR	GLY	THR
3	ALA	GLN	CYS	CYS	ALA	THR	ASP	VAL	LEU	GLY
4	VAL	ALA	ASP	LEU	ASP	CYS	ALA	ASN	PRO	PRO
5	ALA	THR	LEU	ALA	ASN	ALA	THR	HIS	PHE	GLU
6	SER	THR	CYS	ALA	ALA	ILE	GLY	GLN	ARG	ALA
7	ARG	CYS	CYS	VAL	LEU	PRO	ILE	LEU	GLY	GLN
8	ASP	ILE	LEU	CYS	GLN	THR	PRO	ALA	GLY	LEU