BMRB Entry 18324

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18324

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Title:
Backbone Resonance Assignments of the Micro-RNA Interaction Region of Human TRBP2
Deposition date:
2012-03-12
Original release date:
2012-09-14
Authors:
Benoit, Matthieu; Plevin, Michael
Citation:

Citation: Benoit, Matthieu P M H; Plevin, Michael. "Backbone resonance assignments of the micro-RNA precursor binding region of human TRBP."  Biomol. NMR Assignments 7, 229-233 (2013).
PubMed: 22875687

Assembly members:

Assembly members:
TAR-RNA_binding_protein_2_(TRBP2)_(19-228), polymer, 211 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLX06

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TAR-RNA_binding_protein_2_(TRBP2)_(19-228): GIEQMLAANPGKTPISLLQE YGTRIGKTPVYDLLKAEGQA HQPNFTFRVTVGDTSCTGQG PSKKAAKHKAAEVALKHLKG GSMLEPALEDSSSFSPLDSS LPEDIPVFTAAAAATPVPSV VLTRSPAMELQPPVSPQQSE CNPVGALQELVVQKGWRLPE YTVTQESGPAHRKEFTMTCR VERFIEIGSGTSKKLAKRNA AAKMLLRVHTV

Data sets:
Data typeCount
13C chemical shifts589
15N chemical shifts192
1H chemical shifts192

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TAR-RNA binding protein 2 (19-228)1

Entities:

Entity 1, TAR-RNA binding protein 2 (19-228) 211 residues - Formula weight is not available

Glycine 1 comes from the TEV cleavage site

1   GLYILEGLUGLNMETLEUALAALAASNPRO
2   GLYLYSTHRPROILESERLEULEUGLNGLU
3   TYRGLYTHRARGILEGLYLYSTHRPROVAL
4   TYRASPLEULEULYSALAGLUGLYGLNALA
5   HISGLNPROASNPHETHRPHEARGVALTHR
6   VALGLYASPTHRSERCYSTHRGLYGLNGLY
7   PROSERLYSLYSALAALALYSHISLYSALA
8   ALAGLUVALALALEULYSHISLEULYSGLY
9   GLYSERMETLEUGLUPROALALEUGLUASP
10   SERSERSERPHESERPROLEUASPSERSER
11   LEUPROGLUASPILEPROVALPHETHRALA
12   ALAALAALAALATHRPROVALPROSERVAL
13   VALLEUTHRARGSERPROALAMETGLULEU
14   GLNPROPROVALSERPROGLNGLNSERGLU
15   CYSASNPROVALGLYALALEUGLNGLULEU
16   VALVALGLNLYSGLYTRPARGLEUPROGLU
17   TYRTHRVALTHRGLNGLUSERGLYPROALA
18   HISARGLYSGLUPHETHRMETTHRCYSARG
19   VALGLUARGPHEILEGLUILEGLYSERGLY
20   THRSERLYSLYSLEUALALYSARGASNALA
21   ALAALALYSMETLEULEUARGVALHISTHR
22   VAL

Samples:

sample_1: potassium phosphate 23.5 mM; potassium chloride 100 mM; magnesium chloride 10 mM; beta-mercaptoethanol 5 mM; TAR-RNA binding protein 2 (19-228), [U-99% 13C; U-99% 15N; U-99.8% 2H], 600 uM

sample_2: potassium phosphate 23.5 mM; potassium chloride 100 mM; magnesium chloride 10 mM; tris(2-carboxyethyl)phosphine 2 mM; TAR-RNA binding protein 2 (19-228), [U-99% 15N], 300 uM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D BEST TROSY HNCACBsample_1isotropicsample_conditions_1
3D BEST TROSY HN(CO)CACBsample_1isotropicsample_conditions_1
3D BEST TROSY HNCOsample_1isotropicsample_conditions_1
2D 1H-15N BEST TROSY HSQCsample_1isotropicsample_conditions_1
2D 1H-15N-BEST TROSY HSQCsample_2isotropicsample_conditions_1

Software:

ANALYSIS v2.1.5, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian NMRS 600 MHz
  • Varian NMRS 800 MHz

Related Database Links:

UNP Q15633
DBJ BAF83426 BAG73796
GB AAA36765 AAB50581 AAH05860 AAI22654 AAP35804
PIR A38430
REF NP_001069146 NP_004169 NP_599150 NP_599151 XP_002752581
SP Q0IIG6 Q15633
TPG DAA30027
AlphaFold Q9BRY2 Q0IIG6 Q15633

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks