BMRB Entry 18297

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PDB ID: 2lq7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18297
MolProbity Validation Chart

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Title:
E2 binding surface on Uba3 beta-grasp domain undergoes a conformational transition
Deposition date:
2012-02-27
Original release date:
2012-08-29
Authors:
Elgin, Emine; Peterson, Francis; Volkman, Brian
Citation:

Citation: Elgin, E. Sonay; Sokmen, Nazl; Peterson, Francis; Volkman, Brian; Da, Cada; Haas, Arthur. "E2-binding surface on Uba3 -grasp domain undergoes a conformational transition."  Proteins 80, 2482-2487 (2012).
PubMed: 22821745

Assembly members:

Assembly members:
Uba3, polymer, 97 residues, 10658.173 Da.

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Uba3: GSQLPQNIQFSPSAKLQEVL DYLTNSASLQMKSPAITATL EGKNRTLYLQSVTSIEERTR PNLSKTLKELGLVDGQELAV ADVTTPQTVLFKLHFTS

Data sets:
Data typeCount
13C chemical shifts367
15N chemical shifts82
1H chemical shifts591

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Uba31

Entities:

Entity 1, Uba3 97 residues - 10658.173 Da.

The N-terminal GS dipeptide is a cloning artifact.

1   GLYSERGLNLEUPROGLNASNILEGLNPHE
2   SERPROSERALALYSLEUGLNGLUVALLEU
3   ASPTYRLEUTHRASNSERALASERLEUGLN
4   METLYSSERPROALAILETHRALATHRLEU
5   GLUGLYLYSASNARGTHRLEUTYRLEUGLN
6   SERVALTHRSERILEGLUGLUARGTHRARG
7   PROASNLEUSERLYSTHRLEULYSGLULEU
8   GLYLEUVALASPGLYGLNGLULEUALAVAL
9   ALAASPVALTHRTHRPROGLNTHRVALLEU
10   PHELYSLEUHISPHETHRSER

Related Database Links:

PDB 1R4M 1R4N 1TT5 1Y8X 1YOV 2LQ7 2NVU 3DBH 3DBL 3DBR 3FN1 3GZN
DBJ BAA33144 BAC27905 BAC33258 BAE35036 BAE38920
EMBL CAB55996 CAG38568 CAH90059 CAH92113 CAH92482
GB AAC27323 AAC27648 AAH02002 AAH22853 AAH80776
REF NP_001069042 NP_001104576 NP_001126234 NP_001128861 NP_001252890
SP Q5R4A0 Q8C878 Q8TBC4 Q99MI7
TPG DAA17089
AlphaFold Q5R4A0 Q8C878 Q8TBC4 Q99MI7

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