BMRB Entry 18262

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PDB ID: 2lpj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18262
MolProbity Validation Chart

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Title:
NMR structure of major ampullate spidroin 1 N-terminal domain at pH 7.2
Deposition date:
2012-02-14
Original release date:
2012-06-26
Authors:
Jaudzems, Kristaps; Nordling, Kerstin; Landreh, Michael; Rising, Anna; Askarieh, Gelareh; Knight, Stefan; Johansson, Jan
Citation:

Citation: Jaudzems, Kristaps; Askarieh, Glareh; Landreh, Michael; Nordling, Kerstin; Hedhammar, My; Jornvall, Hans; Rising, Anna; Knight, Stefan; Johansson, Jan. "pH-dependent dimerization of spider silk N-terminal domain requires relocation of a wedged tryptophan side chain."  J. Mol. Biol. 422, 477-487 (2012).
PubMed: 22706024

Assembly members:

Assembly members:
Major_ampullate_spidroin_1, polymer, 137 residues, 14183.802 Da.

Natural source:

Natural source:   Common Name: Spiders   Taxonomy ID: 332052   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Euprosthenops australis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: not applicable

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Major_ampullate_spidroin_1: GSGNSHTTPWTNPGLAENFM NSFMQGLSSMPGFTASQLDD MSTIAQSMVQSIQSLAAQGR TSPNKLQALNMAFASSMAEI AASEEGGGSLSTKTSSIASA MSNAFLQTTGVVNQPFINEI TQLVSMFAQAGMNDVSA

Data sets:
Data typeCount
13C chemical shifts418
15N chemical shifts142
1H chemical shifts868

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Major_ampullate_spidroin_11

Entities:

Entity 1, Major_ampullate_spidroin_1 137 residues - 14183.802 Da.

1   GLYSERGLYASNSERHISTHRTHRPROTRP
2   THRASNPROGLYLEUALAGLUASNPHEMET
3   ASNSERPHEMETGLNGLYLEUSERSERMET
4   PROGLYPHETHRALASERGLNLEUASPASP
5   METSERTHRILEALAGLNSERMETVALGLN
6   SERILEGLNSERLEUALAALAGLNGLYARG
7   THRSERPROASNLYSLEUGLNALALEUASN
8   METALAPHEALASERSERMETALAGLUILE
9   ALAALASERGLUGLUGLYGLYGLYSERLEU
10   SERTHRLYSTHRSERSERILEALASERALA
11   METSERASNALAPHELEUGLNTHRTHRGLY
12   VALVALASNGLNPROPHEILEASNGLUILE
13   THRGLNLEUVALSERMETPHEALAGLNALA
14   GLYMETASNASPVALSERALA

Related Database Links:

BMRB 18261 18480
PDB 2LPI 2LPJ 2LTH 3LR2 3LR6 3LR8 3LRD 4FBS
EMBL CAJ90517

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