BMRB Entry 18255

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PDB ID: 2lpd
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18255
MolProbity Validation Chart

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Title:
Solution structure of a MbtH-like protein from Burkholderia pseudomallei, the etiological agent responsible for melioidosis. Seattle Structural Genomics Center for Infectious Disease (SSGCID) target BupsA.13472.b.
Deposition date:
2012-02-10
Original release date:
2012-03-19
Authors:
Buchko, Garry
Citation:

Citation: Buchko, Garry; Hewitt, Stephan; Napuli, Alberto; Van Voorhis, Wesley; Myler, Peter. "Solution structure of a MbtH-like protein from Burkholderia pseudomallei, the etiological agent responsible for melioidosis"  .

Assembly members:

Assembly members:
entity, polymer, 95 residues, 10805.033 Da.

Natural source:

Natural source:   Common Name: Burkholderia pseudomallei   Taxonomy ID: 28450   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Burkholderia pseudomallei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: AVA0421

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MAHHHHHHMGTLEAQTQGPG SMDDELYFVVRNNEGQYSVW MDGRSLPAGWETVGEPATKQ QCLQRIEQLWTDMVPASVRE HLNQHSGPGIDYAVR

Data sets:
Data typeCount
13C chemical shifts339
15N chemical shifts84
1H chemical shifts517

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1MbtH-like protein1

Entities:

Entity 1, MbtH-like protein 95 residues - 10805.033 Da.

The first 21 residues, MAHHHHHHMGTLEAQTQGPGS-,are non-native residue used to facilitate protein purification.

1   METALAHISHISHISHISHISHISMETGLY
2   THRLEUGLUALAGLNTHRGLNGLYPROGLY
3   SERMETASPASPGLULEUTYRPHEVALVAL
4   ARGASNASNGLUGLYGLNTYRSERVALTRP
5   METASPGLYARGSERLEUPROALAGLYTRP
6   GLUTHRVALGLYGLUPROALATHRLYSGLN
7   GLNCYSLEUGLNARGILEGLUGLNLEUTRP
8   THRASPMETVALPROALASERVALARGGLU
9   HISLEUASNGLNHISSERGLYPROGLYILE
10   ASPTYRALAVALARG

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SPARKY: Backbone or all simulated peaks