BMRB Entry 18197

Name: rat Angiogenin
Published: 2012-05-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18197

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

rat Angiogenin

Deposition date:

2012-01-16

Original release date:

2012-05-11

Authors:

Lee, Chung-Kyung; Cheong, Hae-Kap

Citation:

Citation: Lee, Chung-Kyung; Yeo, Kwon Joo; Hwang, Eunha; Cheong, Hae-Kap. "(1)H, (13)C, (15)N backbone and side-chain resonance assignments of rat angiogenin." Biomol. NMR Assignments 7, 89-92 (2013).
PubMed: 22477091

Assembly members:

Assembly members:
angiogenin, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
angiogenin: GSHMAQDDPRYTKFLTQHYD AKPKGRDARYCESMMRRRGL TSPCKEVNTFIHGNKGSIKA ICGANGSPYGENLRISQSPF QITTCKHTGGSPRPPCRYRA SAGFRHVVIACENGLPVHFD ESFISL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	482
15N chemical shifts	123
1H chemical shifts	767

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	angiogenin	1

Entities:

Entity 1, angiogenin 126 residues - Formula weight is not available

1

GLY

SER

HIS

MET

ALA

GLN

ASP

PRO

ARG

2

TYR

THR

LYS

PHE

LEU

THR

GLN

HIS

TYR

ASP

3

ALA

LYS

PRO

LYS

GLY

ARG

ASP

ALA

ARG

TYR

4

CYS

GLU

SER

MET

ARG

GLY

LEU

5

THR

SER

PRO

CYS

LYS

GLU

VAL

ASN

THR

PHE

6

ILE

HIS

GLY

ASN

LYS

GLY

SER

ILE

LYS

ALA

7

ILE

CYS

GLY

ALA

ASN

GLY

SER

PRO

TYR

GLY

8

GLU

ASN

LEU

ARG

ILE

SER

GLN

SER

PRO

PHE

9

GLN

ILE

THR

CYS

LYS

HIS

THR

GLY

10

SER

PRO

ARG

PRO

CYS

ARG

TYR

ARG

ALA

11

SER

ALA

GLY

PHE

ARG

HIS

VAL

ILE

ALA

12

CYS

GLU

ASN

GLY

LEU

PRO

VAL

HIS

PHE

ASP

13

GLU

SER

PHE

ILE

SER

LEU

Samples:

sample_1: angiogenin, [U-100% 13C; U-100% 15N], 1.0 mM; MES 50 mM; sodium chloride 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker DRX 500 MHz

PDB	4QFI 4QFJ
GB	AAR28758 AAV87193 EDL88436 EDL88438
REF	NP_001006993 XP_006251936
TPE	CDG32031