BMRB Entry 18194

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18194
MolProbity Validation Chart

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Title:
1H, 13C, and 15N chemical shift assignments for the 72-residue N-terminal domain of Myxococcus xanthus CarD
Deposition date:
2012-01-12
Original release date:
2013-11-11
Authors:
Jimenez, Angeles; Padmanabhan, S.; Santoro, Jorge
Citation:

Citation: Mirassou, Yasmina; Gallego-Garcia, Aranzazu; Garcia-Heras, Francisco; Garcia-Moreno, Diana; Murillo, Francisco; Elias-Arnanz, Monserrat; Jimenez, Angeles; Padmanabhan, S.. "Solution NMR structure of the 72-residue N-terminal domain of Myxococcus xanthus CarD"  .

Assembly members:

Assembly members:
CarD1-72, polymer, 75 residues, 7879.1 Da.

Natural source:

Natural source:   Common Name: Myxococcus xanthus   Taxonomy ID: 34   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Myxococcus xanthus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTYB12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CarD1-72: AGHMPEGSASLQLAVGDRVV YPNQGVCRVSAIDVKEVAGQ KLTFVTMRREEDGAVVMVPE GKVLAIGVRKVASAE

Data sets:
Data typeCount
13C chemical shifts296
15N chemical shifts73
1H chemical shifts506

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CarD1-721

Entities:

Entity 1, CarD1-72 75 residues - 7879.1 Da.

AGH is the N-terminal cloning tag

1   ALAGLYHISMETPROGLUGLYSERALASER
2   LEUGLNLEUALAVALGLYASPARGVALVAL
3   TYRPROASNGLNGLYVALCYSARGVALSER
4   ALAILEASPVALLYSGLUVALALAGLYGLN
5   LYSLEUTHRPHEVALTHRMETARGARGGLU
6   GLUASPGLYALAVALVALMETVALPROGLU
7   GLYLYSVALLEUALAILEGLYVALARGLYS
8   VALALASERALAGLU

Samples:

sample_1: CarD1-72 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.01%; beta-mercaptoethanol 2 mM; DSS 0.5 mM; H2O 90%; D2O 10%

sample_2: CarD1-72 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.01%; beta-mercaptoethanol 2 mM; DSS 0.5 mM; D2O 100%

sample_3: CarD1-72, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.01%; beta-mercaptoethanol 2 mM; DSS 0.5 mM; H2O 90%; D2O 10%

sample_4: CarD1-72, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.01%; beta-mercaptoethanol 2 mM; DSS 0.5 mM; D2O 100%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HBHA(CO)NHsample_3isotropicsample_conditions_1
3D HBHANHsample_3isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_4isotropicsample_conditions_1
3D HCCH-TOCSYsample_4isotropicsample_conditions_1
3D 1H-13C NOESYsample_4isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
EMBL CAA91224
GB ABF89657 AEI68999 AKQ64384
REF WP_002634407 WP_011555576 WP_013942069

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks