BMRB Entry 18186

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18186
MolProbity Validation Chart

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Title:
Solution structure of the Get5 carboxyl domain from S. cerevisiae
Deposition date:
2012-01-08
Original release date:
2012-02-10
Authors:
Chartron, Justin; VanderVelde, David; Rao, Meera; Clemons, William
Citation:

Citation: Chartron, Justin; VanderVelde, David; Rao, Meera; Clemons, William. "Get5 carboxyl-terminal domain is a novel dimerization motif that tethers an extended Get4/Get5 complex."  J. Biol. Chem. 287, 8310-8317 (2012).
PubMed: 22262836

Assembly members:

Assembly members:
Get5, polymer, 64 residues, 4690.318 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-33b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Get5: SVDPTISKEPEAEKSTNSPA PAPPQELTVPWDDIEALLKN NFENDQAAVRQVMERLQKGW SLAK

Data sets:
Data typeCount
13C chemical shifts270
15N chemical shifts65
1H chemical shifts427

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Get5, chain 11
2Get5, chain 21

Entities:

Entity 1, Get5, chain 1 64 residues - 4690.318 Da.

1   SERVALASPPROTHRILESERLYSGLUPRO
2   GLUALAGLULYSSERTHRASNSERPROALA
3   PROALAPROPROGLNGLULEUTHRVALPRO
4   TRPASPASPILEGLUALALEULEULYSASN
5   ASNPHEGLUASNASPGLNALAALAVALARG
6   GLNVALMETGLUARGLEUGLNLYSGLYTRP
7   SERLEUALALYS

Samples:

sample_1: Get5, [U-100% 13C; U-100% 15N], 3.5 mM; sodium phosphate 20 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_3: Get5, [U-100% 15N], 2.5 mM; sodium phosphate 20 mM; sodium azide 0.02%; polyacrylamide 4%; H2O 90%; D2O 10%

sample_2: Get5, [U-100% 15N], 2.5 mM; sodium phosphate 20 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3anisotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

TOPSPIN, Bruker Biospin - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

ARIA v2.3, Linge, O'Donoghue and Nilges - structure solution

REDCAT, Prestegard - data analysis

CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAA88151 CAA99130 CAY86179
GB AJT73283 AJT77714 AJT80165 AJT87045 AJT92827
REF NP_014530
SP Q12285
TPG DAA10672
AlphaFold Q12285

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks