BMRB Entry 18175

Name: 40-residue D23N beta amyloid fibril
Published: 2012-02-06

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PDB ID: 2lnq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18175
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

40-residue D23N beta amyloid fibril

Deposition date:

2012-01-03

Original release date:

2012-02-06

Authors:

Qiang, Wei; Yau, Wai-Ming; Luo, Yongquan; Mattson, Mark; Tycko, Robert

Citation:

Citation: Qiang, Wei; Yau, Wai-Ming; Luo, Yongquan; Mattson, Mark; Tycko, Robert. "Antiparallel -sheet architecture in Iowa-mutant -amyloid fibrils." Proc. Natl. Acad. Sci. U.S.A. 109, 4443-4448 (2012).
PubMed: 22403062

Assembly members:

Assembly members:
entity, polymer, 26 residues, 2650.167 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: QKLVFFAENVGSNKGAIIGL MVGGVV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	74

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	D23N beta amyloid fibril, chain 1	1
2	D23N beta amyloid fibril, chain 2	1
3	D23N beta amyloid fibril, chain 3	1
4	D23N beta amyloid fibril, chain 4	1
5	D23N beta amyloid fibril, chain 5	1
6	D23N beta amyloid fibril, chain 6	1
7	D23N beta amyloid fibril, chain 7	1
8	D23N beta amyloid fibril, chain 8	1

Entities:

Entity 1, D23N beta amyloid fibril, chain 1 26 residues - 2650.167 Da.

1

GLN

LYS

LEU

VAL

PHE

ALA

GLU

ASN

VAL

2

GLY

SER

ASN

LYS

GLY

ALA

ILE

GLY

LEU

3

MET

VAL

GLY

VAL

Samples:

sample_1: entity, [U-13C], 25 uM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-13C fpRFDR	sample_1	solid	sample_conditions_1
2D 13C-13C RAD	sample_1	solid	sample_conditions_1
2D 13C-13C CHHC	sample_1	solid	sample_conditions_1
1D 13C PITHIRDs-CT	sample_1	solid	sample_conditions_1

Software:

X-PLOR NIH v2.28.1, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

Varian InfinityPlus 400 MHz