BMRB Entry 18108

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PDB ID: 2lme
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18108
MolProbity Validation Chart

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Title:
13C, 15N solid-state MAS NMR chemical shift assignment for YadA membrane anchor domain.
Deposition date:
2011-11-29
Original release date:
2012-11-09
Authors:
Shahid, Shakeel; Markovic, Stefan; Bardiaux, Benjamin; Habeck, Michael; Linke, Dirk; van Rossum, Barth-Jan
Citation:

Citation: Shahid, Shakeel; Markovic, Stefan; Linke, Dirk; van Rossum, Barth-Jan. "Assignment and secondary structure of the YadA membrane protein by solid-state MAS NMR."  Sci. Rep. 2, 803-803 (2012).
PubMed: 23150774

Assembly members:

Assembly members:
YadA_membrane_anchor_domain, polymer, 105 residues, 11200 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pUC-A-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YadA_membrane_anchor_domain: GDQASWSHPQFEKGAHKFRQ LDNRLDKLDTRVDKGLASSA ALNSLFQPYGVGKVNFTAGV GGYRSSQALAIGSGYRVNES VALKAGVAYAGSSDVMYNAS FNIEW

Data sets:
Data typeCount
13C chemical shifts408
15N chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
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Assembly:

Entity Assembly IDEntity NameEntity ID
1YadA_membrane_anchor_domain_11
2YadA_membrane_anchor_domain_21
3YadA_membrane_anchor_domain_31

Entities:

Entity 1, YadA_membrane_anchor_domain_1 105 residues - 11200 Da.

Residues 1-15 belong non native strep tag which was introduced.

1   GLYASPGLNALASERTRPSERHISPROGLN
2   PHEGLULYSGLYALAHISLYSPHEARGGLN
3   LEUASPASNARGLEUASPLYSLEUASPTHR
4   ARGVALASPLYSGLYLEUALASERSERALA
5   ALALEUASNSERLEUPHEGLNPROTYRGLY
6   VALGLYLYSVALASNPHETHRALAGLYVAL
7   GLYGLYTYRARGSERSERGLNALALEUALA
8   ILEGLYSERGLYTYRARGVALASNGLUSER
9   VALALALEULYSALAGLYVALALATYRALA
10   GLYSERSERASPVALMETTYRASNALASER
11   PHEASNILEGLUTRP

Related Database Links:

PDB 2LME
EMBL CAA32085 CAA32086 CAL10087 CBW54734 CBY78119
GB AAK69254 AAN37524 ADZ44497 AJI81101 AJJ21461
REF NP_783714 NP_863557 WP_005176545 WP_011100755 WP_011117647
SP A1JUB7 P0C2W0 P31489
AlphaFold A1JUB7 P0C2W0 P31489