BMRB Entry 18031

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the ribosoaml assembly factor Fap7 from Pyrococcus horikoshii in its AP5A bound form
Published: 2012-11-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18031

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the ribosoaml assembly factor Fap7 from Pyrococcus horikoshii in its AP5A bound form

Deposition date:

2011-10-31

Original release date:

2012-11-09

Authors:

Hellmich, Ute; Duchardt-Ferner, Elke; Woehnert, Jens

Citation:

Citation: Hellmich, Ute; Duchardt-Ferner, Elke; Woehnert, Jens. "1H, 15N, 13C Assigned Backbone Chemical Shifts for Fap7 in the AP5A-bound form" Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
Fap7, polymer, 180 residues, 20178.1 Da.
AP5, non-polymer, 916.367 Da.

Natural source:

Natural source: Common Name: Pyrococcus horikoshii Taxonomy ID: 53953 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus horikoshii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fap7: MLIAITGTPGVGKTTVAKLL AKKLNYEYVSLKDFALEKGC GRRVNDEVEVEIDELAYFIE RELKGKNAVLDGHLSHLMPV DLVVVLRAHPKLIGERLRER GYDREKIGENVEAELVDAVL IEAIEEHENVIEVDTTNKSP EDVVEEIVSLINSGIKKRVG IVDWSEVYDEIIPYLRLGGE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	319
15N chemical shifts	155
1H chemical shifts	155

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Fap7	1
2	AP5A	2

Entities:

Entity 1, Fap7 180 residues - 20178.1 Da.

1	MET	LEU	ILE	ALA	ILE	THR	GLY	THR	PRO	GLY
2	VAL	GLY	LYS	THR	THR	VAL	ALA	LYS	LEU	LEU
3	ALA	LYS	LYS	LEU	ASN	TYR	GLU	TYR	VAL	SER
4	LEU	LYS	ASP	PHE	ALA	LEU	GLU	LYS	GLY	CYS
5	GLY	ARG	ARG	VAL	ASN	ASP	GLU	VAL	GLU	VAL
6	GLU	ILE	ASP	GLU	LEU	ALA	TYR	PHE	ILE	GLU
7	ARG	GLU	LEU	LYS	GLY	LYS	ASN	ALA	VAL	LEU
8	ASP	GLY	HIS	LEU	SER	HIS	LEU	MET	PRO	VAL
9	ASP	LEU	VAL	VAL	VAL	LEU	ARG	ALA	HIS	PRO
10	LYS	LEU	ILE	GLY	GLU	ARG	LEU	ARG	GLU	ARG
11	GLY	TYR	ASP	ARG	GLU	LYS	ILE	GLY	GLU	ASN
12	VAL	GLU	ALA	GLU	LEU	VAL	ASP	ALA	VAL	LEU
13	ILE	GLU	ALA	ILE	GLU	GLU	HIS	GLU	ASN	VAL
14	ILE	GLU	VAL	ASP	THR	THR	ASN	LYS	SER	PRO
15	GLU	ASP	VAL	VAL	GLU	GLU	ILE	VAL	SER	LEU
16	ILE	ASN	SER	GLY	ILE	LYS	LYS	ARG	VAL	GLY
17	ILE	VAL	ASP	TRP	SER	GLU	VAL	TYR	ASP	GLU
18	ILE	ILE	PRO	TYR	LEU	ARG	LEU	GLY	GLY	GLU

Entity 2, AP5A - C20 H29 N10 O22 P5 - 916.367 Da.

1

AP5

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

DBJ	BAA30356
REF	WP_010885344
SP	O58998
AlphaFold	O58998

BMRB Entry 18031

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	LEU	ILE	ALA	ILE	THR	GLY	THR	PRO	GLY
2	VAL	GLY	LYS	THR	THR	VAL	ALA	LYS	LEU	LEU
3	ALA	LYS	LYS	LEU	ASN	TYR	GLU	TYR	VAL	SER
4	LEU	LYS	ASP	PHE	ALA	LEU	GLU	LYS	GLY	CYS
5	GLY	ARG	ARG	VAL	ASN	ASP	GLU	VAL	GLU	VAL
6	GLU	ILE	ASP	GLU	LEU	ALA	TYR	PHE	ILE	GLU
7	ARG	GLU	LEU	LYS	GLY	LYS	ASN	ALA	VAL	LEU
8	ASP	GLY	HIS	LEU	SER	HIS	LEU	MET	PRO	VAL
9	ASP	LEU	VAL	VAL	VAL	LEU	ARG	ALA	HIS	PRO
10	LYS	LEU	ILE	GLY	GLU	ARG	LEU	ARG	GLU	ARG
11	GLY	TYR	ASP	ARG	GLU	LYS	ILE	GLY	GLU	ASN
12	VAL	GLU	ALA	GLU	LEU	VAL	ASP	ALA	VAL	LEU
13	ILE	GLU	ALA	ILE	GLU	GLU	HIS	GLU	ASN	VAL
14	ILE	GLU	VAL	ASP	THR	THR	ASN	LYS	SER	PRO
15	GLU	ASP	VAL	VAL	GLU	GLU	ILE	VAL	SER	LEU
16	ILE	ASN	SER	GLY	ILE	LYS	LYS	ARG	VAL	GLY
17	ILE	VAL	ASP	TRP	SER	GLU	VAL	TYR	ASP	GLU
18	ILE	ILE	PRO	TYR	LEU	ARG	LEU	GLY	GLY	GLU

1	MET	LEU	ILE	ALA	ILE	THR	GLY	THR	PRO	GLY
2	VAL	GLY	LYS	THR	THR	VAL	ALA	LYS	LEU	LEU
3	ALA	LYS	LYS	LEU	ASN	TYR	GLU	TYR	VAL	SER
4	LEU	LYS	ASP	PHE	ALA	LEU	GLU	LYS	GLY	CYS
5	GLY	ARG	ARG	VAL	ASN	ASP	GLU	VAL	GLU	VAL
6	GLU	ILE	ASP	GLU	LEU	ALA	TYR	PHE	ILE	GLU
7	ARG	GLU	LEU	LYS	GLY	LYS	ASN	ALA	VAL	LEU
8	ASP	GLY	HIS	LEU	SER	HIS	LEU	MET	PRO	VAL
9	ASP	LEU	VAL	VAL	VAL	LEU	ARG	ALA	HIS	PRO
10	LYS	LEU	ILE	GLY	GLU	ARG	LEU	ARG	GLU	ARG
11	GLY	TYR	ASP	ARG	GLU	LYS	ILE	GLY	GLU	ASN
12	VAL	GLU	ALA	GLU	LEU	VAL	ASP	ALA	VAL	LEU
13	ILE	GLU	ALA	ILE	GLU	GLU	HIS	GLU	ASN	VAL
14	ILE	GLU	VAL	ASP	THR	THR	ASN	LYS	SER	PRO
15	GLU	ASP	VAL	VAL	GLU	GLU	ILE	VAL	SER	LEU
16	ILE	ASN	SER	GLY	ILE	LYS	LYS	ARG	VAL	GLY
17	ILE	VAL	ASP	TRP	SER	GLU	VAL	TYR	ASP	GLU
18	ILE	ILE	PRO	TYR	LEU	ARG	LEU	GLY	GLY	GLU

1	MET	LEU	ILE	ALA	ILE	THR	GLY	THR	PRO	GLY
2	VAL	GLY	LYS	THR	THR	VAL	ALA	LYS	LEU	LEU
3	ALA	LYS	LYS	LEU	ASN	TYR	GLU	TYR	VAL	SER
4	LEU	LYS	ASP	PHE	ALA	LEU	GLU	LYS	GLY	CYS
5	GLY	ARG	ARG	VAL	ASN	ASP	GLU	VAL	GLU	VAL
6	GLU	ILE	ASP	GLU	LEU	ALA	TYR	PHE	ILE	GLU
7	ARG	GLU	LEU	LYS	GLY	LYS	ASN	ALA	VAL	LEU
8	ASP	GLY	HIS	LEU	SER	HIS	LEU	MET	PRO	VAL
9	ASP	LEU	VAL	VAL	VAL	LEU	ARG	ALA	HIS	PRO
10	LYS	LEU	ILE	GLY	GLU	ARG	LEU	ARG	GLU	ARG
11	GLY	TYR	ASP	ARG	GLU	LYS	ILE	GLY	GLU	ASN
12	VAL	GLU	ALA	GLU	LEU	VAL	ASP	ALA	VAL	LEU
13	ILE	GLU	ALA	ILE	GLU	GLU	HIS	GLU	ASN	VAL
14	ILE	GLU	VAL	ASP	THR	THR	ASN	LYS	SER	PRO
15	GLU	ASP	VAL	VAL	GLU	GLU	ILE	VAL	SER	LEU
16	ILE	ASN	SER	GLY	ILE	LYS	LYS	ARG	VAL	GLY
17	ILE	VAL	ASP	TRP	SER	GLU	VAL	TYR	ASP	GLU
18	ILE	ILE	PRO	TYR	LEU	ARG	LEU	GLY	GLY	GLU