BMRB Entry 18026

Name: Backbone Chemical Shift Assignments for Unmyristoylated GCAP1 bound to Ca2+ ions
Published: 2012-03-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18026

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Chemical Shift Assignments for Unmyristoylated GCAP1 bound to Ca2+ ions

Deposition date:

2011-10-28

Original release date:

2012-03-09

Authors:

Lim, Sunghyuk; Ames, James

Citation:

Citation: Lim, Sunghyuk; Peshenko, Igor; Dizhoor, Alexander; Ames, James. "Backbone (1)H, (13)C, and (15)N resonance assignments of guanylyl cyclase activating protein-1, GCAP1." Biomol. NMR Assignments 7, 39-42 (2013).
PubMed: 22392341

Assembly members:

Assembly members:
GCAP1, polymer, 205 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Cattle Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GCAP1: MGNIMDGKSVEELSSTECHQ WYKKFMTECPSGQLTLYEFR QFFGLKNLSPWASQYVEQMF ETFDFNKDGYIDFMEYVAAL SLVLKGKVEQKLRWYFKLYD VDGNGCIDRDELLTIIRAIR AINPCSDSTMTAEEFTDTVF SKIDVNGDGELSLEEFMEGV QKDQMLLDTLTRSLDLTRIV RRLQNGEQDEEGASGRETEA AEADG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	326
15N chemical shifts	131
1H chemical shifts	131

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GCAP1	1
2	Calcium ions 1	2
3	Calcium ions 2	2
4	Calcium ions 3	2

Entities:

Entity 1, GCAP1 205 residues - Formula weight is not available

1

MET

GLY

ASN

ILE

MET

ASP

GLY

LYS

SER

VAL

2

GLU

LEU

SER

THR

GLU

CYS

HIS

GLN

3

TRP

TYR

LYS

PHE

MET

THR

GLU

CYS

PRO

4

SER

GLY

GLN

LEU

THR

LEU

TYR

GLU

PHE

ARG

5

GLN

PHE

GLY

LEU

LYS

ASN

LEU

SER

PRO

6

TRP

ALA

SER

GLN

TYR

VAL

GLU

GLN

MET

PHE

7

GLU

THR

PHE

ASP

PHE

ASN

LYS

ASP

GLY

TYR

8

ILE

ASP

PHE

MET

GLU

TYR

VAL

ALA

LEU

9

SER

LEU

VAL

LEU

LYS

GLY

LYS

VAL

GLU

GLN

10

LYS

LEU

ARG

TRP

TYR

PHE

LYS

LEU

TYR

ASP

11

VAL

ASP

GLY

ASN

GLY

CYS

ILE

ASP

ARG

ASP

12

GLU

LEU

THR

ILE

ARG

ALA

ILE

ARG

13

ALA

ILE

ASN

PRO

CYS

SER

ASP

SER

THR

MET

14

THR

ALA

GLU

PHE

THR

ASP

THR

VAL

PHE

15

SER

LYS

ILE

ASP

VAL

ASN

GLY

ASP

GLY

GLU

16

LEU

SER

LEU

GLU

PHE

MET

GLU

GLY

VAL

17

GLN

LYS

ASP

GLN

MET

LEU

ASP

THR

LEU

18

THR

ARG

SER

LEU

ASP

LEU

THR

ARG

ILE

VAL

19

ARG

LEU

GLN

ASN

GLY

GLU

GLN

ASP

GLU

20

GLU

GLY

ALA

SER

GLY

ARG

GLU

THR

GLU

ALA

21

ALA

GLU

ALA

ASP

GLY

Entity 2, Calcium ions 1 - Ca - 40.078 Da.

1

CA

Samples:

sample_1: TRIS, [U-100% 2H], 5 mM; DTT, [U-100% 2H], 5 mM; H2O 93%; D2O, [U-100% 2H], 7%; GCAP1, [U-100% 15N], 0.7 mM

sample_2: TRIS, [U-100% 2H], 5 mM; DTT, [U-100% 2H], 5 mM; H2O 93%; D2O, [U-100% 2H], 7%; GCAP1, [U-100% 13C; U-100% 15N], 0.7 mM

sample_3: TRIS, [U-100% 2H], 5 mM; DTT, [U-100% 2H], 5 mM; H2O 93%; D2O, [U-100% 2H], 7%; GCAP1, [U-13C; U-15N; U-2H], 0.7 mM

sample_conditions_1: ionic strength: 30 mM; pH: 7.4; pressure: 1 atm; temperature: 317 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D HN(CO)CA	sample_3	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_3	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz

EMBL	CAA64642
GB	AAB31698
REF	NP_776971 XP_003482237 XP_004408126 XP_005969718 XP_006069254
SP	P46065
TPG	DAA16514
AlphaFold	P46065