BMRB Entry 18016

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PDB ID: 2lky
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18016
MolProbity Validation Chart

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Title:
Solution structure of MSMEG_1053, the second DUF3349 annotated protein in the genome of Mycobacterium smegmatis. Seattle Structural Genomics Center for Infectious Disease (SSGCID) target MysmA.17112.b
Deposition date:
2011-10-22
Original release date:
2011-11-29
Authors:
Buchko, Garry
Citation:

Citation: Buchko, Garry; Edwards, Thomas; Abendroth, Jan; Napuli, Alberto; Hewitt, Stephen; Phan, Isabelle; Van Voorhis, Wesley; Kim, Chang-Yub; Myler, Peter. "Different structures for two DUF3349 annotated proteins in the genome of Mycobacterium smegmatis suggest a structural diversity within the DUF3349 superfamily."  .

Assembly members:

Assembly members:
entity, polymer, 112 residues, 12434.074 Da.

Natural source:

Natural source:   Common Name: High GC Gram+   Taxonomy ID: 1772   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium smegmatis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: AVA0421

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GPGSMVNAFLAKIAAWLNAG YPEGVPGPDRVPLLALLTRR LTNDEIKAIAEDLEKRAHFD HIDIGVLITQMTDEMPREED IERVRRHLALQGWPLDDPRD GEEPDGESGGPR

Data sets:
Data typeCount
13C chemical shifts453
15N chemical shifts108
1H chemical shifts744

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1SSGCID1

Entities:

Entity 1, SSGCID 112 residues - 12434.074 Da.

Residues 1-4 represent non-native residues that remain after the removal of an N-terminal affinity tag.

1   GLYPROGLYSERMETVALASNALAPHELEU
2   ALALYSILEALAALATRPLEUASNALAGLY
3   TYRPROGLUGLYVALPROGLYPROASPARG
4   VALPROLEULEUALALEULEUTHRARGARG
5   LEUTHRASNASPGLUILELYSALAILEALA
6   GLUASPLEUGLULYSARGALAHISPHEASP
7   HISILEASPILEGLYVALLEUILETHRGLN
8   METTHRASPGLUMETPROARGGLUGLUASP
9   ILEGLUARGVALARGARGHISLEUALALEU
10   GLNGLYTRPPROLEUASPASPPROARGASP
11   GLYGLUGLUPROASPGLYGLUSERGLYGLY
12   PROARG

Related Database Links:

PDB 2LKY
EMBL CKH10149
GB ABK71419 AFP37510 AIU06313 AIU12938 AIU19562
REF WP_003892430 YP_885459

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