BMRB Entry 18002

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PDB ID: 2lko
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18002
MolProbity Validation Chart

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Title:
Structural Basis of Phosphoinositide Binding to Kindlin-2 Pleckstrin Homology Domain in Regulating Integrin Activation
Deposition date:
2011-10-17
Original release date:
2011-11-21
Authors:
Liu, Jianmin; Fukuda, Koichi; Xu, Zhen
Citation:

Citation: Liu, Jianmin; Fukuda, Koichi; Xu, Zhen; Ma, Yan-Qing; Hirbawi, Jamila; Mao, Xian; Wu, Chuanyue; Plow, Edward; Qin, Jun. "Structural basis of phosphoinositide binding to kindlin-2 protein pleckstrin homology domain in regulating integrin activation."  J. Biol. Chem. 286, 43334-43342 (2011).
PubMed: 22030399

Assembly members:

Assembly members:
Kindlin-2, polymer, 138 residues, 15722.256 Da.
4IP, non-polymer, 500.075 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Kindlin-2: GSHMGDITSIPELADYIKVF KPKKLTLKGYKQYWCTFKDT SISCYKSKEESSGTPAHQMN LRGCEVTPDVNISGQKFNIK LLIPVAEGMNEIWLRCDNEK QYAHWMAACRLASKGKTMAD SSYNLEVQNILSFLKMQH

Data sets:
Data typeCount
13C chemical shifts377
15N chemical shifts129
1H chemical shifts669

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Kindlin-21
24IP2

Entities:

Entity 1, Kindlin-2 138 residues - 15722.256 Da.

1   GLYSERHISMETGLYASPILETHRSERILE
2   PROGLULEUALAASPTYRILELYSVALPHE
3   LYSPROLYSLYSLEUTHRLEULYSGLYTYR
4   LYSGLNTYRTRPCYSTHRPHELYSASPTHR
5   SERILESERCYSTYRLYSSERLYSGLUGLU
6   SERSERGLYTHRPROALAHISGLNMETASN
7   LEUARGGLYCYSGLUVALTHRPROASPVAL
8   ASNILESERGLYGLNLYSPHEASNILELYS
9   LEULEUILEPROVALALAGLUGLYMETASN
10   GLUILETRPLEUARGCYSASPASNGLULYS
11   GLNTYRALAHISTRPMETALAALACYSARG
12   LEUALASERLYSGLYLYSTHRMETALAASP
13   SERSERTYRASNLEUGLUVALGLNASNILE
14   LEUSERPHELEULYSMETGLNHIS

Entity 2, 4IP - C6 H16 O18 P4 - 500.075 Da.

1   4IP

Related Database Links:

PDB 2LKO 4F7H
DBJ BAE38850 BAF84427
EMBL CAA80852 CAD61925
GB AAH17327 AAH33436 AAH83876 AAI51293 AAN75823
REF NP_001011915 NP_001094734 NP_001128471 NP_001128472 NP_001253248
SP Q8CIB5 Q96AC1
TPG DAA25234
AlphaFold Q8CIB5 Q96AC1

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