BMRB Entry 17965

Name: Solution NMR structure of Alr2454 protein from Nostoc sp. strain PCC 7120, Northeast Structural Genomics Consortium Target NsR264
Published: 2011-10-24

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PDB ID: 2ljw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17965
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of Alr2454 protein from Nostoc sp. strain PCC 7120, Northeast Structural Genomics Consortium Target NsR264

Deposition date:

2011-09-30

Original release date:

2011-10-24

Authors:

Aramini, James; Lee, Dong yup; Ciccosanti, Colleen; Janjua, Haleema; Rost, Burkhard; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano

Citation:

Citation: Aramini, James; Lee, Dong yup; Ciccosanti, Colleen; Janjua, Haleema; Rost, Burkhard; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano. "Solution NMR structure of Alr2454 protein from Nostoc sp. strain PCC 7120, Northeast Structural Genomics Consortium Target NsR264" To be published ., .-..

Assembly members:

Assembly members:
NsR264, polymer, 110 residues, 12163.996 Da.

Natural source:

Natural source: Common Name: Nostoc sp. Taxonomy ID: 1180 Superkingdom: Bacteria Kingdom: not available Genus/species: Nostoc sp.

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21_NESG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NsR264: MTGQELRQLLLDKWGYSYDV QFRRTQGKIFLQVMWKYLEQ ASFPMNETEYQEHLDSVANY LHALGGAVQVKTFITQTKER PRLGKAVSIPLDLGERASEW IILEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	500
15N chemical shifts	119
1H chemical shifts	790

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NsR264	1

Entities:

Entity 1, NsR264 110 residues - 12163.996 Da.

C-terminal LEHHHHHH purification tag

1	MET	THR	GLY	GLN	GLU	LEU	ARG	GLN	LEU	LEU
2	LEU	ASP	LYS	TRP	GLY	TYR	SER	TYR	ASP	VAL
3	GLN	PHE	ARG	ARG	THR	GLN	GLY	LYS	ILE	PHE
4	LEU	GLN	VAL	MET	TRP	LYS	TYR	LEU	GLU	GLN
5	ALA	SER	PHE	PRO	MET	ASN	GLU	THR	GLU	TYR
6	GLN	GLU	HIS	LEU	ASP	SER	VAL	ALA	ASN	TYR
7	LEU	HIS	ALA	LEU	GLY	GLY	ALA	VAL	GLN	VAL
8	LYS	THR	PHE	ILE	THR	GLN	THR	LYS	GLU	ARG
9	PRO	ARG	LEU	GLY	LYS	ALA	VAL	SER	ILE	PRO
10	LEU	ASP	LEU	GLY	GLU	ARG	ALA	SER	GLU	TRP
11	ILE	ILE	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	NsR264_007	isotropic	sample_conditions_1
2D 1H-13C HSQC	NsR264_007	isotropic	sample_conditions_1
3D 1H-15N NOESY	NsR264_007	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	NsR264_007	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	NsR264_007	isotropic	sample_conditions_1
2D 1H-13C HSQC high resolution (L/V methyl stereoassignment)	NsR264_008	isotropic	sample_conditions_1
3D HNCO	NsR264_007	isotropic	sample_conditions_1
3D HN(CA)CO	NsR264_007	isotropic	sample_conditions_1
3D HNCA	NsR264_007	isotropic	sample_conditions_1
3D HN(CO)CA	NsR264_007	isotropic	sample_conditions_1
3D CBCA(CO)NH	NsR264_007	isotropic	sample_conditions_1
3D HNCACB	NsR264_007	isotropic	sample_conditions_1
3D HBHA(CO)NH	NsR264_007	isotropic	sample_conditions_1
3D HCCH-TOCSY	NsR264_007	isotropic	sample_conditions_1
3D CCH-TOCSY	NsR264_007	isotropic	sample_conditions_1
3D HCCH COSY	NsR264_007	isotropic	sample_conditions_1
1D 15N T1 and T2	NsR264_007	isotropic	sample_conditions_1
2D 1H-15N hetNOE	NsR264_007	isotropic	sample_conditions_1

PDB	2LJW
DBJ	BAB74153
GB	ABA20012
REF	WP_010996610

BMRB Entry 17965

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	THR	GLY	GLN	GLU	LEU	ARG	GLN	LEU	LEU
2	LEU	ASP	LYS	TRP	GLY	TYR	SER	TYR	ASP	VAL
3	GLN	PHE	ARG	ARG	THR	GLN	GLY	LYS	ILE	PHE
4	LEU	GLN	VAL	MET	TRP	LYS	TYR	LEU	GLU	GLN
5	ALA	SER	PHE	PRO	MET	ASN	GLU	THR	GLU	TYR
6	GLN	GLU	HIS	LEU	ASP	SER	VAL	ALA	ASN	TYR
7	LEU	HIS	ALA	LEU	GLY	GLY	ALA	VAL	GLN	VAL
8	LYS	THR	PHE	ILE	THR	GLN	THR	LYS	GLU	ARG
9	PRO	ARG	LEU	GLY	LYS	ALA	VAL	SER	ILE	PRO
10	LEU	ASP	LEU	GLY	GLU	ARG	ALA	SER	GLU	TRP
11	ILE	ILE	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	THR	GLY	GLN	GLU	LEU	ARG	GLN	LEU	LEU
2	LEU	ASP	LYS	TRP	GLY	TYR	SER	TYR	ASP	VAL
3	GLN	PHE	ARG	ARG	THR	GLN	GLY	LYS	ILE	PHE
4	LEU	GLN	VAL	MET	TRP	LYS	TYR	LEU	GLU	GLN
5	ALA	SER	PHE	PRO	MET	ASN	GLU	THR	GLU	TYR
6	GLN	GLU	HIS	LEU	ASP	SER	VAL	ALA	ASN	TYR
7	LEU	HIS	ALA	LEU	GLY	GLY	ALA	VAL	GLN	VAL
8	LYS	THR	PHE	ILE	THR	GLN	THR	LYS	GLU	ARG
9	PRO	ARG	LEU	GLY	LYS	ALA	VAL	SER	ILE	PRO
10	LEU	ASP	LEU	GLY	GLU	ARG	ALA	SER	GLU	TRP
11	ILE	ILE	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	THR	GLY	GLN	GLU	LEU	ARG	GLN	LEU	LEU
2	LEU	ASP	LYS	TRP	GLY	TYR	SER	TYR	ASP	VAL
3	GLN	PHE	ARG	ARG	THR	GLN	GLY	LYS	ILE	PHE
4	LEU	GLN	VAL	MET	TRP	LYS	TYR	LEU	GLU	GLN
5	ALA	SER	PHE	PRO	MET	ASN	GLU	THR	GLU	TYR
6	GLN	GLU	HIS	LEU	ASP	SER	VAL	ALA	ASN	TYR
7	LEU	HIS	ALA	LEU	GLY	GLY	ALA	VAL	GLN	VAL
8	LYS	THR	PHE	ILE	THR	GLN	THR	LYS	GLU	ARG
9	PRO	ARG	LEU	GLY	LYS	ALA	VAL	SER	ILE	PRO
10	LEU	ASP	LEU	GLY	GLU	ARG	ALA	SER	GLU	TRP
11	ILE	ILE	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS